Analyses of vasoactive peptides released from the vascular endothelial cells

血管内皮细胞释放的血管活性肽的分析

• 批准号: 03670019
• 负责人: FUJIMOTO Sunao
• 金额: $ 1.02万
• 依托单位: University of Occupational and Environmental Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1991
• 资助国家: 日本
• 起止时间: 1991 至 1992
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-03670019/
• 关键词: Double-immunolabeling; Weibel-Palade body; Human umbilical vein; Immunoelectron microscopy; Toad aorta; Vasocontraction; Endothelin; Histamine; 腹大動脈; WeibelーPalade body; von Willebrand factor; 血管攣縮; 免疫組織化学; 血管内皮

1) Release of two kinds of peptides (20k and 30k) were detected from Weibel-Palade bodies (WP) in the cultured endothelial cells of the full term human umbilical veins after administration of compound 48/80, a histamine releaser. Since these peptides were not obtained from control samples which WP is not degranulated, they are considered to be derived from WP.2) The immunoelectron microscopy using the double-immunolabeling technique on the same ultrathin section revealed that WP of the human umbilical vein endothelial cells shows simultaneous localizations of histamine and von willebrand factor.3) Dose-dependent tension curves were recorded from the in-vitro toad aortar after administration of endothelin-1 and sarafotoxin-S6b. By a single dose application (10-^8M) of these two drugs to both endothelium-preserved and venuded vessels, the induction of the endothelium-dependent vasocontraction occurs after 2 min of administration, and the ultrastructural changes of WP such as degranulation and expulsion of their contents by exocytosis become evident within 2 min. This indicates that WP contains vasocontractile peptides released by these two drugs.

1)用组胺释放剂化合物48/80作用于培养的人脐静脉内皮细胞，检测到两种肽（20 k和30 k）从韦伯-帕拉德小体（WP）中释放出来。由于这些肽不是从WP未脱颗粒的对照样品中获得的，2）在同一切片上用免疫电子显微镜双标记技术显示，人脐静脉内皮细胞的WP同时显示组胺和von willebrand因子的定位。给予内皮素-1和sarafotoxin-S6 b后的离体蟾蜍粪便。将这两种药物单剂量（10-^8M）应用于保存内皮的血管和微血管，在给药2 min后诱导内皮依赖性血管收缩，并且在2 min内WP的超微结构变化如脱颗粒和内容物通过胞吐排出变得明显。这表明WP含有这两种药物释放的血管收缩肽。

项目成果

Yoshitaka SAKAMOTO,Sunao FUJIMOTO他: "Immunoelectron microscopy on the localization of endothelin in the umbilical vein of perinatal rabbits" Anatomical Record.

Yoshitaka SAKAMOTO、Sunao FUJIMOTO 等：“免疫电镜观察围产期兔脐静脉内皮素的定位”解剖记录。

Hiroshi Ueda;Sunao Fujimoto 他: "Simultaneous localization of histamine and factor VIIIーrelated antigen in the endothelium of the human umbillical vein" Anatomical Record. 232. 257-261 (1992)

Hiroshi Ueda；Sunao Fujimoto 等人：“人脐静脉内皮细胞中组胺和因子 VIII 相关抗原的同时定位”解剖记录 232. 257-261 (1992)。

Hiroshi UEDA,Sunao FUJIMOTO他: "Simultaneous localization of histamine and factor VIII-related antigen in the endothelium of the human umbilical vein" Anatomical Record. 232. 257-261 (1992)

Hiroshi UEDA、Sunao FUJIMOTO 等人：“人脐静脉内皮细胞中组胺和因子 VIII 相关抗原的同时定位”解剖记录 232. 257-261 (1992)。

Yoshiaki DOI,Sunao FUJIMOTO: "Ultrastructural changes of Weibel-Palade bodies in the endothelia of toad aortas after administration of endothelin and sarafotoxin" International Journal of Microcirculation:Clinical and Experimental. 11. S23 (1992)

Yoshiaki DOI、Sunao FUJIMOTO：“施用内皮素和 sarafotoxin 后蟾蜍主动脉内皮中 Weibel-Palade 小体的超微结构变化”国际微循环杂志：临床与实验。

Doi,Y. and Fujimoto,S.: "Vasocontractions of the in-vitro toad aortas induced by endothelin-1 and sarafotoxin-S6b" Anatomical Record. 235. 253-260 (1993)

土井，Y.