Analyses of vasoactive peptides released from the vascular endothelial cells

1)在体外培养的足月人脐静脉内皮细胞中，给药组胺释放剂化合物48/80后，检测了webel - palade小体（WP）中两种肽（20k和30k）的释放。由于这些肽不是从未脱颗粒的对照样品中获得的，因此它们被认为来自WP。2)在同一超薄切片上使用双免疫标记技术的免疫电子显微镜显示，人脐静脉内皮细胞的WP同时定位组胺和血管性血液病因子。3)记录内皮素-1和sarafotoxin-S6b对离体蟾蜍主动脉张力的剂量依赖性曲线。将这两种药物单剂量（10-^8M）应用于保存内皮的血管和灌注血管，在给药2分钟后，内皮依赖性血管收缩发生，并在2分钟内发生超微结构变化，如脱粒和胞吐排出其内容物。这表明白蜡含有这两种药物释放的血管收缩肽。

1) Release of two kinds of peptides (20k and 30k) were detected from Weibel-Palade bodies (WP) in the cultured endothelial cells of the full term human umbilical veins after administration of compound 48/80, a histamine releaser. Since these peptides were not obtained from control samples which WP is not degranulated, they are considered to be derived from WP.2) The immunoelectron microscopy using the double-immunolabeling technique on the same ultrathin section revealed that WP of the human umbilical vein endothelial cells shows simultaneous localizations of histamine and von willebrand factor.3) Dose-dependent tension curves were recorded from the in-vitro toad aortar after administration of endothelin-1 and sarafotoxin-S6b. By a single dose application (10-^8M) of these two drugs to both endothelium-preserved and venuded vessels, the induction of the endothelium-dependent vasocontraction occurs after 2 min of administration, and the ultrastructural changes of WP such as degranulation and expulsion of their contents by exocytosis become evident within 2 min. This indicates that WP contains vasocontractile peptides released by these two drugs.