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Immunolocalizations of ET-1, ET receptors and CGRP in endothelial cells

内皮细胞中 ET-1、ET 受体和 CGRP 的免疫定位

基本信息

批准号：
07670044
负责人：
FUJIMOTO Sunao
金额：
$ 1.28万
依托单位：
University of Occupational and Environmental Health
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1995
资助国家：
日本
起止时间：
1995 至 1996
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07670044/
关键词：
endothlin-1 CGRP Weibel-Palade body endothelin receptors enous sinus of dura mater vascular carotid body artery immunocytochemistry endothelial cell エンドセリン-1 CGRP ワイベル・パラ-デ顆粒血管内皮細胞脳硬静脈洞ビック・エンドセリン

项目摘要

Vascular endothelial cells play the improtant roles in regulation of the local blood flow by producing some vasoactive peptides such as endothelin (ET) -1 and calcitonin gene-related peptide (CGRP), and by releasing them into both vascular lumen and subendothelial layr. Our immunoelectron microscopy revealed that ET-1 and CGRP are synthesized and segregated in the rough endoplasmic reticulum-Golgi system, stored in part in Weibel-Palade (WP) bodies in endothelial cells of the transverse venous sinus of the rat dura mater and the artery of the rat carotid body. In addition, we confirmed that both peptides are extracellularly released from the WP bodies into both vascular lumen and subendothelial layr by exocytosis in a manner of regulated pathway.It is widely accepted that EP-1 induces vasoconstriction mediated from ETA receptors on the sarcolemma of medial muscle cells in a manner of paracrine, and vasodilatation mediated from ETB receptors on the plasmalemma of endothelial cells in a manner of autocrine. The present immunocytochemisty showed that immunoreactivities of the ETB receptors are localized to the plasmalemma of endothelial cells of the transverse venous sinus and the artery in the carotid body, and that those of ETA receptors are localized to the sarcolemma of medial muscle cells of the transverse venous sinus. In the arterial system of the carotid body, we could not detect out immunoreactions of the ETA receptors. These findings suggest that ET-1 regulates the blood flow in the transverse sinus by inducing both vasocontraction mediated from ETA receptors and vasodilatation mediated from ETB receptors, and that ET-1 preserves an abundance of the blood flow through the carotid body by evoking the vasodilatation mediated from ETB receptors.

血管内皮细胞通过产生内皮素(ET)-1和降钙素基因相关肽(CGRP)等血管活性多肽，并将其释放到血管腔和内皮下层，在局部血流调节中发挥重要作用。免疫电子显微镜观察到ET-1和CGRP在粗面内质网-高尔基体系统中合成和分离，部分储存在大鼠硬脑膜横静脉窦内皮细胞和大鼠颈动脉的Webel-Palade小体中。此外，我们还证实了这两种多肽都是通过胞吐途径从WP小体外释放到血管腔和内皮下层的。人们普遍认为，EP-1以旁分泌方式诱导内侧肌细胞肌膜上ETA受体介导的血管收缩，以自分泌方式诱导内皮细胞质膜上ETB受体介导的血管扩张。免疫细胞化学结果表明，ETB受体的免疫反应定位于颈动脉体横静脉窦内皮细胞和动脉的胞膜，ETA受体的免疫反应定位于横静脉窦内膜肌细胞的肌膜。在颈动脉体部的动脉系统中，未检测到ETA受体的免疫反应。这些结果表明，ET-1通过ETA受体介导的血管收缩和ETB受体介导的血管扩张来调节横窦的血流量，而ET-1通过激发ETB受体介导的血管扩张来维持大量通过颈动脉小体的血流量。