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Morphological study of shear stress (blood flow rate) loaded artery

剪切应力（血流量）加载动脉的形态学研究

基本信息

批准号：
61570164
负责人：
MASUDA Hirotake
金额：
$ 1.47万
依托单位：
Akita University School of Medicine
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1987
项目状态：
已结题

项目摘要

Morphological changes in the shear stress (blood flow) loaded artery were studied using arterio-venous shunt method following Kamiya and Togawa (Am J phyiol 239:14-21,1980). We used arterio-venous anastomosis between the common carotied artery and the external jugular vein in adult female beagel dogs and rats and observed the proximal portion of the common carotid artery from the anastomosis where blood flow rate was increased. In dogs, endothelial cells showed the protrusion of nucleic parts, appearance of microvilli, disturbance of pinocytotoc vesicles and increased cytoplasmic microfilament bundles (actin filament stress fibers) as early as 1 week. They increased in density (6,000 cells/mmxmm)(3,000 cells/mmxmm at the contralateral common carotid artery) in 4 weeks. They were slender but thick. Their nuclei were prolate spheroid in shape and as big as those of the contralateral control which were oblate spheroid in shape. Bsement membrane of the endothelial cells was thick as early as 1 week. Internal elastic lamina was splitted and multiplicated in 4 weeks. Media showed distinct increase of elastic fiber and elastic lamina in 6 to 12 months. To disclose the process of increase of endothelial cells in 4 weeks, we used bromodeoxyuridine (BrdU). With application of monoclonal antibody to BrdU, we found BrdU incroporation into the endothelial cell nuclei. In rats, almost same endothelial cell changes could be observed in the flow loaded carotid arteries. They increased in dencity (7,000 cells/mmxmm) in 2 to 4 weeks (3,000 cells/mmxmm in contralateral common carotid artery). From this study, it is clear that endothelial cells change actively under the loadance of shear stress (blood flow) and arterial wall changes in chronic stage. It should be important to study vascular research, such as the study of atherogenesis, with a thought that vascular is under the control of shear stress (blood flow).

按照Kamiya和Tokawa(Am J phyiol 239：14-21，1980)，使用动静脉分流方法研究了剪切应力(血流)负载动脉的形态变化。我们对成年雌性比吉犬和大鼠进行颈总动脉与颈外静脉之间的动静脉吻合术，观察吻合处颈总动脉近端部分的血流量增加。在狗中，内皮细胞早在1周时就表现出核部分的突出、微绒毛的出现、胞饮小泡的紊乱以及细胞质微丝束（肌动蛋白丝应力纤维）的增加。 4 周内，它们的密度增加（6,000 个细胞/mmxmm）（对侧颈总动脉为 3,000 个细胞/mmxmm）。它们细长但厚实。它们的细胞核呈扁球体形状，并且与对侧对照的扁球体形状一样大。内皮细胞的基底膜早在1周就已增厚。内弹性层在4周内分裂并增殖。介质在6至12个月内显示出弹性纤维和弹性层的明显增加。为了揭示4周内内皮细胞增加的过程，我们使用了溴脱氧尿苷（BrdU）。通过应用 BrdU 单克隆抗体，我们发现 BrdU 融入内皮细胞核中。在大鼠中，在流量负载的颈动脉中可以观察到几乎相同的内皮细胞变化。 2 至 4 周内，它们的密度增加（7,000 个细胞/mmxmm）（对侧颈总动脉为 3,000 个细胞/mmxmm）。从这项研究中可以清楚地看出，在慢性阶段，内皮细胞在剪切应力（血流）和动脉壁变化的负荷下发生活跃变化。研究血管研究应该很重要，例如动脉粥样硬化的研究，认为血管受到剪切应力（血流）的控制。