Cloning and characterization of ribosomal RNA operon and its application to medicine
Cloning and characterization of ribosomal RNA operon and its application to medicine
批准号:
61570210
负责人:
YAMADA Takeshi
金额:
$1.22万
依托单位国家:
日本
项目类别:
Grant-in-Aid for General Scientific Research (C)
财政年份:
1986
资助国家:
日本
项目状态:
已结题
起止时间:
1986 至 1987
中文摘要
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英文摘要
The number of rRNA genes in Mycobacterium bovis BCG and Streptomyces lividans TK21 was examined by Southern hybridizatrion of labeled 5S, 16S and 23S rRNA with restricted DNA. The results suggested that M. bovis BCG chromosomes may carry only a minimum set of rRNA genes and S. lividans TK21 at least 6. The sequences of 16S rRNA genes, spacers and promoter regions of both organisms were determined. Promoter activity of rRNA operon form both organisms was also examined. The results revealed that Escherichia coli RNA polymerase recognized M. bovis BCG promoter, wheras it did not recognize the S. lividans TK21 promoter. The transcription site of M. bovis BCG rRNA operon was determined by Sl nuclease mapping and primer extension methods.
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Yamada,T.: Microbiology and Immunology. (1987)
山田 T.:微生物学和免疫学。
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Udo, Takezou: "Biochemical Mechanisms of Antibiotics Resistance in a Clinical isolate of Mycobacterium fortuitum: Presence of < beta>-lactamase and Aminoglycoside-acetyltransferase and Possible Particiption of Altered Drug Transport on the Resistance Mech
Udo,Takezou:“偶发分枝杆菌临床分离株抗生素耐药性的生化机制:β-内酰胺酶和氨基糖苷乙酰转移酶的存在以及改变的药物转运可能参与耐药机制
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山田毅: The Actinomycetologist. 49. 10-14 (1986)
山田武:放线菌学家。49. 10-14 (1986)
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Shuzuki, Yasuhiko: "Organization of Ribosomal RNA Genes in Mycobacterium Bovis BCG" Journal of Bacteriology. 169. 839-843 (1987)
Shuzuki、Yasuhiko:“牛分枝杆菌 BCG 中核糖体 RNA 基因的组织”细菌学杂志。
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Udou,T.: Amer.Rev.Resp.Dis.133. 653-657 (1986)
Udou,T.:Amer.Rev.Resp.Dis.133。
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Studies on the pathophysiology and gene therapy for adrenoleukodystrophy using knock-out mice
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Biological Effects of Tritium Radiation Quantitatively Estimated Using Mouse Fetal Cell Damage
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The Development of Chemical Bimetal
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Tritium RBE estimation based on effects of chronic ingestion of tritiaed water on fetal development of mouse
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Design and Development of the Irradiation Chamber for Radiobiological Experiments Using Synchrotron Radiation in the Energy Region 0.4 - 5 keV.
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依托单位:
海外基金