Design and Development of the Irradiation Chamber for Radiobiological Experiments Using Synchrotron Radiation in the Energy Region 0.4 - 5 keV.

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The synchrotron radiation at the photon Factory, National Laboratory for High Energy Physics, is the first major facility dedicated to all kinds of project experiments including radiation biology. The soft X-rays within synchrotron radiation in the energy region 0.4-5 KeV is expected to be one of the most important tools in radiation biology, because the monochromatic soft X-rays can be used as a fine probe of the biologically sensitive structure within cells. However, the soft X-rays can not be used in the conventional radiobiological experiments because most of the radiation energy is absorbed by the air phase.In the present study, we designed and developed the irradiation chamber using such soft X-rays, which enabled us to irradiate the cultured cells with the X-rays in the vacuum or in the helium gas phase. The irradiation samples, together with the whole chamber, can be scanned two dimensionally in the plane vertical to the X-ray beam, to widen the irradiation area as well as to average the intensity distribution. The differential pumping system was installed between the irradiation chamber and the monochrometer in the beam line. Operation of the irradiation experiment can be automatically controlled by a personal computer system.