Establishment of a new method for the diagnosis of differentintestinal diseases by determination of serum bile acids.

我们试图建立一种通过测定血清胆汁酸来鉴别细菌过度生长综合征和吸收不良综合征的新方法。由于游离胆汁酸和脱氧胆酸分别通过去结合作用和肠道细菌的7a脱羟基作用而升高，因此用高效液相色谱法测定单个胆汁酸可以诊断过度生长综合征。另一方面，通过测定口服500 mg熊去氧胆酸后总胆汁酸的血浆清除曲线，可以诊断吸收不良综合征。因此，在存在吸收不良的情况下，清除曲线变平。因此，通过测定口服500 mg熊去氧胆酸后总胆汁酸的血浆清除曲线，可以诊断吸收不良综合征。因此，在存在吸收不良的情况下，清除曲线变平使用这两种测试，可以通过采集血液样本来鉴别诊断过度生长综合征和吸收不良综合征，而这是不可能的。

We tried to establish a new method for the differential diagnosis of the bacterial overgrowth syndrome and the malabsorption syndrome by determining the serum bile acid.By the determination of the individual bile acids with use of high performance of liquid chromatography it was possible to diagnose the overgrowth syndrome,since free bile acid and deoxycholic acid were increased by deconjugation or 7a dehydroxylation by the intestinal bacteria, respectively.On the other hand , by the determination of plasma clearance curvve of total bile acids after oral loading of 500mg ursodexycholic acid it was possible to diagnose the malabsorption syndrome, since clearance curve became flat in the existence of malabsorption.Therefore, with use of both tests it was possible to make differential diagnosis between overgrowth syndrome and malabsorption syndromeby taking blood sample which has been impossible.