权益分类	功能权益	普通用户	{{item.name}}会员
{{category.name}}	{{benefitItem.name}}

Establishment of the chemical diagnosis in intestinal diseases with serum bile acid determination

血清胆汁酸测定肠道疾病化学诊断的建立

基本信息

批准号：
63570308
负责人：
OSUGA Toshiaki
金额：
$ 1.41万
依托单位：
Institute of Clinical Medicine, University of Tsukuba
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1988
资助国家：
日本
起止时间：
1988 至 1989
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-63570308/
关键词：
malabsorption bacterial overgrowth oral bile acid loading test serum bile acid profile unconjugated bile acid dexycholic acid 腸内細菌過剰型増殖腸内細菌過剰発育症候群吸収不良症候群鑑別診断

项目摘要

The increase of unconjugated bile acid (F) or deoxycholic acid (DC) occurred in some gastrointestinal diseases with bacterial overgrowth. The oral UDCA loading test was useful in diagnosing the bile acid malabsorption. Therefore, in order to know the biochemical information of the pathogenesis in the alimentary tract , we tried to distinguish bacterial overgrowth from bile acid malabsorption by combining the determination of serum bile acid profile and oral UDCA loading tests. [Subjects and Methods ] We measured serum bile acid profile in 50 cases with various gastrointestinal disorders and 12 normal controls by HPLC with 3alpha-HSD immobilized column. We performed oral 300mg of UDCA loading test in 16 cases with intestinal diseases. Total serum bile acid was measured by enzymatic fluorometrically. [Results] 1) In 23 out of 52 cases with various gastrointestinal diseases, the percentage of F increased, and in 16 out of 52 cases, DC increased, compared with the one in normal controls. F in the intestinal fluid collected during surgery, increased remarkably as well as the one in serum. 2) In 10 out of 16 cases with intestinal diseases , oral UDCA loading test revealed a significant decrease of exogeneous UDCA compared with the one in normal controls. 3) Using the combination of the oral UDCA loading test and the measurement of serum individual bile acid in 14 cases with intestinal diseases, these could be classified into 4 types as follows ; Type I ; abnormal profile group ( increased F or DC), Type II ; flattened serum bile acid clearance curve, Type III ; both abnormal profile and flattened curve, Type IV ; normal group [Conclusion] The bacterial overgrowth and bile acid malabsorption could be differentiated with using the combination of the measurement of serum bile acid profile and the oral UDCA loading test.

非结合胆汁酸（F）或脱氧胆酸（DC）的增加发生在一些细菌过度生长的胃肠道疾病中。口服 UDCA 负荷试验有助于诊断胆汁酸吸收不良。因此，为了了解消化道发病机制的生化信息，我们试图通过结合血清胆汁酸谱测定和口服UDCA负荷试验来区分细菌过度生长和胆汁酸吸收不良。 [对象和方法] 采用3α-HSD固定柱高效液相色谱法测定了50例各种胃肠道疾病患者和12例正常对照者的血清胆汁酸谱。我们对16例肠道疾病患者进行了口服300mg UDCA负荷试验。通过酶荧光法测量总血清胆汁酸。 [结果]1）与正常对照相比，52例各种胃肠道疾病患者中，F百分比升高23例，52例中DC升高16例。手术中收集的肠液中的F与血清中的F一样显着升高。 2）16例肠道疾病患者中，有10例口服UDCA负荷试验显示外源性UDCA较正常对照显着减少。 3）结合口服UDCA负荷试验和血清个体胆汁酸测定，将14例肠道疾病患者分为以下4种类型： I型；轮廓异常组（F 或 DC 增加），II 型；血清胆汁酸清除率曲线平坦，III 型；轮廓异常且曲线平坦，IV 型；【结论】血清胆汁酸谱测定与口服UDCA负荷试验相结合，可区分细菌过度生长和胆汁酸吸收不良。