Establishment of Acquired Immune Deficiency Mouse Model, and Its Mycobacterium intracellulare Infection.

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Recently many patients with acquired immune deficiency syndrome (AIDS) were reported to have frequent Mycobacterium intracellulare infection. But the treatment of both of them were quite unsatisfactory. We have established the M.intracellulare infection mouse model, and by using it, have investigated about the treatment of its infection further. For the purpose of establish the immune deficiency model, nomoclonal anti-mouse Thy1.2 antibody was administered to the M.intracellulare infection model mice. but unsatisfied immune depression was earned. So we decided to investigate the inflammatory cell dynamics for the mycobacterial infection at first. Inflammatory cells were recovered by total pulmonary lavage (TPL) and stained with monoclonal anti-Thy1.2, Lyt-2(CD8), L3T4(CD4), and Ia. The cell surface antigen expression were measured by flow cytometrical cell analizer. Tpls were performed during the course of infection. Thy1.2 positive and Lyt-2 positive lymphocytes were increased soon after the infection, but the L3T4 positive lymphocytes and Ia positive macrophages were gradually increased three weeks after infection. Thy1.2 positive lymphocytes and Ia positive macrophages reached the plateau six weeks after infection. On the contraty, Lyt-2 positive and L3T4 positive lymphocytes were up and down with their surface antigen expression ratio and have a contra-rotation course as if they were feed back controlled each other. For the next step, the effects of biological response modifiers (BRMs) were investigated. Recombinant interleukin-2 (rIL-2), Muramyl dipeptide derivative; DJ-7041 and cyclosporin A (CyA) were administered to the M.intracellulare infection mice. The increase of Ia positive macrophages at the early stage after infection by rIL-2 treated mice and at the late stage by DJ-7041 treated mice were observed. By using this model we are going to establish the immune deficiency mouse model akin to the AIDS with M.intracellulare infection.