Simultaneous measurements of cerebral blood flow and energy metabolism by 19F and 31P nuclear magnetic resonance spectroscopy.

为建立脑血流量（CBF）和能量代谢的在体核磁共振（NMR）测量方法，对JEOL-JNM-GX-270，6.34Tesla的在体NMR谱仪进行了改进，使其能自动获得血清19 F或1HNMR谱。通过对D20和19 F氟里昂（三氟甲烷）清除率的测定，发现D20注射后脑内1H NMR信号短暂降低，随后逐渐恢复到注射前水平。用~（19）F氟里昂吸入长爪沙鼠10 min后，用~（19）F核磁共振波谱仪记录了~（19）F氟里昂在长爪沙鼠脑内的饱和和去饱和情况。根据19 F氟里昂的去饱和曲线估计CBF。从D20清除率或19 F氟里昂清除率获得的CBF值与通过以下方法估计的流量值相关良好：结果表明，吸入5%CO_2时CBF较对照组增加30%，双侧颈总动脉阻断时CBF较对照组减少90%以上。因此，使用体内1H或19 F NMR光谱，测量CBF已成为可能。该系统允许同时测量31 P NMR光谱。利用该系统研究了脑血流量与能量代谢的关系，同时测定了脑和肌肉中Na ~+和水的含量。

The study was performed to develop method for measuring cerebral blood flow (CBF) and energy metabolism simultaneously by in vivo hmrlear magnetic resonance (NMR) spectroscopy.In vivo NMR spectrometer (JEOL-JNM-GX-270,6.34 Tesla) was modified,so that seroal 19F or 1H NMR spectra were obtained automatically. Using this system,deuterium oxide (D20) clearance or 19F freon (trifluoromethane) clearance was recorded.D20 was injected as a bolus from common carotid artery of mongolian gerbils,and 1H NMR signals were monitored serially in the gerbil brain.1H signals in the brain were reduced transiently following the D20 injection and thereafter recovered gradually towards the pre-injection level. The CBF was Estimatid from this clearance curve.19F freon was inhaled also in mongolian gerbils for about 10 min,and its saturation and desaturation in the gerbil brain were recorded by serial 19F NMR spectroscopy.19F freon has small toxicity providing little influence on arterial blood pressure,blood gases and EEG.The CBF was estimated from the desaturation curve of 19F freon.The CBF values obtained from D20 clearance or 19F freon clearance correlated well with flow values estimated by conventional 1jC-iodoantipyrine methods.The CBF measured during 5% CO2 inhalation was increased by 30% compared with that mesured during control state.The CBF measured during bilateral common carotid artery occlusion was reduced by more than 90% compared with the control values. Thus,using in vivo 1H or 19F NMR spectroscopy,measurement of CBF has become possible.This system allows to measure 31P NMR spectroscopy simultaneously. Therefore,simultaneous measurements of CBF and energy metabolism are possible by this technique.Utilizing this system,the relationship between CBF and energy metabolism was examined.Likewise,measurements of intra-and extracellular na+ and water in the brain and muscle were carried out.