Simultaneous measurements of cerebral blood flow and energy metabolism by 19F and 31P nuclear magnetic resonance spectroscopy.

本研究旨在建立体内核磁共振（NMR）光谱同时测量脑血流量（CBF）和能量代谢的方法。对体内核磁共振谱仪（JEOL-JNM-GX-270,6.34 Tesla）进行了改进，使其能够自动获得多个19F或1H核磁共振谱。使用该系统，记录了氧化氘（D20）清除率或19F氟利昂（三氟甲烷）清除率。从蒙古沙鼠颈总动脉注射D20，连续监测沙鼠脑内1H NMR信号。D20注射后，脑内1H信号短暂降低，随后逐渐恢复至注射前水平。CBF是根据这个间隙曲线估计的。同时对蒙古沙鼠吸入19F氟利昂约10 min，用序列19F NMR谱仪记录其在沙鼠脑内的饱和和去饱和状态。氟利昂毒性小，对动脉血压、血气和脑电图影响不大。根据19F氟利昂的去饱和曲线估计了CBF。从D20间隙或19F氟利昂间隙获得的CBF值与传统的1jc -碘安替比林方法估计的流量值具有良好的相关性。与对照组相比，吸入5% CO2时测得的CBF增加了30%。与对照组相比，双侧颈总动脉闭塞期间测量的CBF降低了90%以上。因此，使用体内1H或19F核磁共振光谱，测量CBF已成为可能。该系统允许同时测量31P核磁共振波谱。因此，通过这种技术可以同时测量CBF和能量代谢。利用该系统，研究了脑血流与能量代谢的关系。同样，测量了大脑和肌肉中细胞内和细胞外的na+和水。

The study was performed to develop method for measuring cerebral blood flow (CBF) and energy metabolism simultaneously by in vivo hmrlear magnetic resonance (NMR) spectroscopy.In vivo NMR spectrometer (JEOL-JNM-GX-270,6.34 Tesla) was modified,so that seroal 19F or 1H NMR spectra were obtained automatically. Using this system,deuterium oxide (D20) clearance or 19F freon (trifluoromethane) clearance was recorded.D20 was injected as a bolus from common carotid artery of mongolian gerbils,and 1H NMR signals were monitored serially in the gerbil brain.1H signals in the brain were reduced transiently following the D20 injection and thereafter recovered gradually towards the pre-injection level. The CBF was Estimatid from this clearance curve.19F freon was inhaled also in mongolian gerbils for about 10 min,and its saturation and desaturation in the gerbil brain were recorded by serial 19F NMR spectroscopy.19F freon has small toxicity providing little influence on arterial blood pressure,blood gases and EEG.The CBF was estimated from the desaturation curve of 19F freon.The CBF values obtained from D20 clearance or 19F freon clearance correlated well with flow values estimated by conventional 1jC-iodoantipyrine methods.The CBF measured during 5% CO2 inhalation was increased by 30% compared with that mesured during control state.The CBF measured during bilateral common carotid artery occlusion was reduced by more than 90% compared with the control values. Thus,using in vivo 1H or 19F NMR spectroscopy,measurement of CBF has become possible.This system allows to measure 31P NMR spectroscopy simultaneously. Therefore,simultaneous measurements of CBF and energy metabolism are possible by this technique.Utilizing this system,the relationship between CBF and energy metabolism was examined.Likewise,measurements of intra-and extracellular na+ and water in the brain and muscle were carried out.