Studies on the enhancement of maxrophage killing by the selective activation of their tumoricidal activity and tumor cell modi-ication

为了研究活化的巨噬细胞对肿瘤细胞的破坏作用，我们研究了肿瘤细胞对巨噬细胞结合和细胞溶解的敏感性增强，以及选择性活化灭瘤巨噬细胞。0.1KE/ml的OK-432预处理肿瘤细胞2小时后，活化的巨噬细胞对MM102和MH-134肿瘤细胞的杀伤活性显著增强。此外，经OK-432预处理后的肿瘤细胞表面结构未发生变化，活化的巨噬细胞对OK-432预处理后的肿瘤细胞的结合和细胞溶解能力明显增强，表明通过对肿瘤细胞进行修饰，增强了巨噬细胞杀伤活性。为了进一步分析BRM活化巨噬细胞的作用机制，我们研究了摄取fitc偶联的OK-432后巨噬细胞分离后表面结构的形态学变化。注射FITC-OK432的小鼠腹腔渗出细胞中巨噬细胞的数量为39.3%，未注射OK-432的小鼠为17.0%。细胞内携带OK432的细胞在巨噬细胞中占15.8%，在淋巴细胞中占2.1%。细胞内携带OK432的巨噬细胞在表面结构上表现出发育良好的褶边和片状足，类似于杀瘤巨噬细胞。

To develop the tumor cell destruction by activated macrophages, the enhancement of tumor cell susceptibility to macrophage binding and cytolysis, and the selective activation of tumoricidal macrophages were investigated. Tumoricidal activity of activated macrophages against MM102 and MH-134 tumor cells were significantly enhanced when tumor cells were pretreated with 0.1KE/ml of OK-432 for 2 hr. Furthermore, these tumor cells pretreated with OK-432 showed no changes on their surface structure by the SEM examination and binding and cytolysis of activated macrophages against OK-432 pretreated tumor cells were significantly enhanced, suffesting that macrophage killing activities were enhanced by the modification of tumor cells.For the further analysis of action mechamisms of macrophage activation by BRM, morphological changes of surface structure of macropahges fractionated with the uptake of FITC-conjugated OK-432 were investigated. Macrophage population among peritoneal exsudate cells was 39.3% in mice treated with ip injection of FITC-OK432, while that was 17.0% without OK-432 injection. The populations of cells with intracelluar OK432 were 15.8% in macrophage fraction amd 2.1% in lymphocyte one, respectively. Macrophages with intracelluar OK432 showed well developed ruffles and the appearance of lamellipodia on their surface structure, resembling tumoricidal macrophages.