Diode laser modulation of wound healing and laser-assisted venous anastomosis

(1)为了评估激光对创面愈合的调节作用，我们在大鼠背部制作全层皮肤缺损，周围包裹聚四氟乙烯环，每天用脉冲GaAlAs激光照射。能量密度为0.6/cm的fe辐照后，肉芽组织的干重、1型和3型胶原明显增加，粒细胞和巨噬细胞明显减少。结果表明，脉冲GaAlAs激光通过促进胶原合成和抑制胶原变性来促进创面愈合。为了证明我们新研制的二极管激光装置在微血管端到端吻合中的有效性，我们对大鼠腹主动脉进行了野生化，并与常规缝合吻合进行了比较。在该装置中，二极管激光能量被传递到接触陶瓷尖端。我们评估了24周后的长期随访观察，并检查了组织学结果和吻合的机械强度。我们证实，这种新的直接接触方法的发展，从而使我们能够准确和容易地照射组织。

(1). To assess laser modulation of wound healing, full thickness skin defects sorrounded by Teflon rings were made on the back of rats and were irradiated with pulsed GaAlAs laser every day. Dry weight, type 1 and type 3 collagen were markedly increased, and granulocytes and macrophages of granulation tissue were decreased on 10 p.o.d.by thfe irradiation at an energy density of 0.6/cm. The results suggested that pulsed GaAlAs laser stimulates wound healing by promoting collagen synthesis and inhibiting collagen degeneration.(2). To demonstrate the usefulness of our newly produced diode laser device for microvascular end-to-end anastomoses, we wilded Rat' abdominal aorta and compared with conventional suture anastomosis. In the device, diode laser energy is delivered to a contact ceramics tip. We assesed the long term follow-up observation up to 24 weeks later and examined both histological findings and mechanical intensity of the anastomosis. We confirm that the development of this new direct contact method thus enables us to irradiate the tissue exactly and easily.