Diode laser modulation of wound healing and laser-assisted venous anastomosis

二极管激光调制伤口愈合和激光辅助静脉吻合术

• 批准号: 61570606
• 负责人: SAKAGUCHI Shukichi
• 金额: $ 1.34万
• 依托单位: Hamamatsu university school of medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570606/
• 关键词: diode laser; wound healing; in situ hybridization method; oncogene; small hepatocellular carcinoma; 内膜側温度; 1.半導体パルスレーザー; コラーゲン; 炎症 2.レーザー血管吻合; 半導体パルスレーザー; 炎症抑制

(1). To assess laser modulation of wound healing, full thickness skin defects sorrounded by Teflon rings were made on the back of rats and were irradiated with pulsed GaAlAs laser every day. Dry weight, type 1 and type 3 collagen were markedly increased, and granulocytes and macrophages of granulation tissue were decreased on 10 p.o.d.by thfe irradiation at an energy density of 0.6/cm. The results suggested that pulsed GaAlAs laser stimulates wound healing by promoting collagen synthesis and inhibiting collagen degeneration.(2). To demonstrate the usefulness of our newly produced diode laser device for microvascular end-to-end anastomoses, we wilded Rat' abdominal aorta and compared with conventional suture anastomosis. In the device, diode laser energy is delivered to a contact ceramics tip. We assesed the long term follow-up observation up to 24 weeks later and examined both histological findings and mechanical intensity of the anastomosis. We confirm that the development of this new direct contact method thus enables us to irradiate the tissue exactly and easily.

（一）.为了评估激光对伤口愈合的调节，在大鼠背部制作由聚四氟乙烯环环绕的全层皮肤缺损，每天用脉冲GaAlAs激光照射。p.o.d.by能量密度为0.6/cm ~ 2的10次照射下，肉芽组织的干重、1型和3型胶原显著增加，而粒细胞和巨噬细胞减少。实验结果表明，脉冲GaAlAs激光通过促进胶原合成和抑制胶原变性促进创面愈合。（二）、为了证明我们新生产的半导体激光装置在微血管端端吻合中的有效性，我们将大鼠腹主动脉野生化并与传统的缝合吻合进行比较。在该装置中，二极管激光能量被输送到接触陶瓷尖端。我们评估了24周后的长期随访观察，并检查了吻合口的组织学结果和机械强度。我们证实，这种新的直接接触方法的发展，从而使我们能够准确和容易地照射组织。

项目成果

海野直樹: 脈管学. (1989)

海野直树：血管学（1989）。

Junko Mori: "In situ hybridization of DHBV DNA in experimental infection" Acta Hepatologica Japonica. 27. 1418-1423 (1986)

Junko Mori：“实验感染中 DHBV DNA 的原位杂交”Acta Hepatologica Japonica。

Naoki Unno: Lasers in Surgery and Medicine. (1989)

Naoki Unno：激光在外科和医学中的应用。

石原康守: 日本レーザー医学会論文抄録集. 200 (1988)

Yasumori Ishihara：日本激光医学学会文摘 200 (1988)。

Naoki Unno: "Laser-induced experimental abdominal acrtic aneurysms in rats conseguence of wide diode laser irradiation" Journal of Vascular Surgery.

Naoki Unno：“宽二极管激光照射引起的大鼠实验性腹主动脉瘤”，《血管外科杂志》。