Effect of Inhibition of the Amino Acid Transport on Metabolism of the Lens.

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The defect of amino acids in the lens may affect the metabolism and cause to alterations of the structure and functions of lenses. The purpose of this project is investigate the effect of amino acid deficiency on function of the lens. 1. Gamma-glutamyl cycle play a important role on amino acid transport to lens. 2. The amino acid transport to lens wa decreased with aging, but free amino acid concentration was increased. By adding inhibitors of <gamm>-glutamyl cycle enzymes to cultured lensees, free amino acid concentration was also increased. 3. The activity of lens LDH was not affected by inhibitors, yet <gamm>-GTP was inhibited. 4. Effect of inhibitors of amino acid transport was not found out in in vivo experiments. But it was suspected that effect of aqueous humor collection lead lens cloudy. It was suggested that lowering of amino acid transport in lens epithels was related of lens function by lowering amino acids uptake and generation of cataract should be dissolved. From the result of in vivo experiment, chemical and physical changes of aqueous humor (e.g. content and change of tension) also should be considered.