Study on rat hypothalamus arcuate nucleus which plays an important role in the induction of acupuncture analgesia.

大鼠下丘脑弓状核在针刺镇痛诱导中起重要作用的研究

• 批准号: 61570893
• 负责人: HAMBA Michiko
• 金额: $ 1.15万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1986
• 资助国家: 日本
• 起止时间: 1986 至 1987
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-61570893/
• 关键词: arcuate nucleus; electroacupuncture analgesia; Opioids; 針刺激

To evaluate the possible role of the arcuate nucleus of hypothalamus (ARH) in the antinociceptive system, the following experiments were carried out, using male Wistar rats. 1) The effect of ARH lesion on pain control and electroacupuncture (EA) analgesia. The ARH-lesioned rats showed a significantly high value in pain rating of the formalin test 30 days after, indicating that they were hypersensitive to nociceptive stimuli. An analgesic effect was induced in the control rats by EA stimulation (3 Hz, 300-600 <micr>A) applied at Ho-Ku, but scarcely seen in the lesioned rats. 2) EA effect on the neuronal activity of ARH. The majority of ARH neurons significantly changed their spontaneous frequency rates (the rate increased or decreased) by EA stimulation. The activity change lasted for about 60 min and its time course agreed with that of the analgesic effect of EA. It suggested that the majority of ARH neurons took part in the analgesia. 3) Relationship between tha analgesic effect of the EA and the possible involvement of endogenous opioids. The analgesic effects and the neuronsl change in ARH induced by EA stimulation were both antagonized by injected naloxone (1mg/Kg, i.p.).<bat>-endorphin in the medialbasal hypothalamus was significantly higher in the animal group immediately killed after reciving EA stimulation for 15 min than in the control animal group. 4) The effect of ARH stimulation on the neuronal activity of the lateral hypothalamic area (LHA). ARH stimulation (20-45 <micr>A, 1-3 Hz, 30 s) induced the suppressive effect on the spontaneous activity of the neurons in the LHA and on the response of the LHA neurons to noxious stimuli. ARH resion thus appears to be an important part in the central pain control system and in the induction of EA analgesia.

为了评价下丘脑弓状核（ARH）在抗伤害性感受系统中的可能作用，用雄性Wistar大鼠进行了下列实验。1)损毁ARH对疼痛控制及电针镇痛的影响ARH损伤大鼠在30天后的福尔马林试验中显示出显著高的疼痛等级值，表明它们对伤害性刺激过敏。电针（3 Hz，300-600 A）可引起对照组大鼠的镇痛作用<micr>，而电针损伤组大鼠几乎未见镇痛作用。2)电针对ARH神经元活动的影响。电针刺激后，大多数ARH神经元的自发频率发生显著变化（频率增加或降低）。这种活动变化持续约60 min，其时程与电针镇痛作用的时程一致。提示大部分ARH神经元参与镇痛作用。3)电针镇痛作用与内源性阿片类物质的关系。腹腔注射纳洛酮（1 mg/Kg）可拮抗电针引起的大鼠ARH的镇痛作用和神经元的变化。<bat>电针15 min后立即处死的动物组下丘脑内侧基底区的β-内啡肽含量明显高于对照组。4)刺激ARH对下丘脑外侧区（LHA）神经元活动的影响。ARH刺激（20-45 <micr>A，1-3 Hz，30 s）可抑制LHA神经元的自发活动和对伤害性刺激的反应。因此，ARH反应似乎是中枢疼痛控制系统和EA镇痛诱导的重要组成部分。

项目成果

半場道子,戸田一雄: 昭和歯学会雑誌. 7. 266 (1987)

Michiko Hanba，Kazuo Toda：《昭和牙科学会杂志》7. 266 (1987)。

Michiko Hamba: "The effects of the arcuate uncleus lesion on pain control and on electroacupuncture analgeia." J. Showa Univ. Dent. Soci.8. (1988)

Michiko Hamba：“弓形叔叔病变对疼痛控制和电针镇痛的影响。”

Hamba,M;Toda,K.: J.Physiol.Soc.Japan. 46. 456 (1984)

Hamba，M；Toda，K.：日本生理学会杂志。

Hamba,M;Toda,K: Experimental Neurology. 87. 118-128 (1985)

Hamba，M；Toda，K：实验神经病学。