Effects of orofacial pain on mastication estimated by expression of c-fos-like immunoreactivity in the rat trigeminal caudalis.

通过大鼠三叉神经尾肌中 c-fos 样免疫反应性的表达来评估口面部疼痛对咀嚼的影响。

• 批准号: 07838035
• 负责人: HAMBA Michiko
• 金额: $ 1.66万
• 依托单位: Showa University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1995
• 资助国家: 日本
• 起止时间: 1995 至 1996
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-07838035/
• 关键词: proto-oncogen; c-tos-immunoreactive; Interleukin-IB; trigeminal caudalis; sustained pain; orthodontic stimulation; periodontal disease; Glutamate-receptor; Proto-Oncogene; Interleukin-1B; Interleukin; NSAIDs

1 The expression of c-fos-like immunoreactivity (For-IR) was studied in the trigeminal caudalis of the chloralose anesthetized Wister rats after injection of 0.05 ug of interleukin-1-bata (IL-1b) into the gingiva of the incisor or application of the orthodontical mechanical stimulation. IL-1b injection-induced Fos-IR (IL-1b) was suppressed by administration of non-steroidal anti-inflammatory drugs (NSAIds) at the early period postinjection. The results suggest that a small amount of IL-1b at the site of periodontal disease can induce Fos-IR in pain-processing neurons.2 The effects of the antagonists and agonists of glutamate receptors on the expression of Fos-IR were examined after tooth pulp stimulation. MK-801, CNQX,AP-3, NMDA,AMPA and phorbol ester were intrathecally administered to rats 10 min prior to stimulation (0.2 ms, 0.6 mA,0.5 Hz, 10 min) ; the effects or each agent on the expression of Fos-IR was studied in the caudalis at 2 h post-stimulation. The results suggest that Ca^<2+> entry through an NMDA receptor is crucial to the induction of c-Fos and to the development of persistent pain.

1观察切牙牙周注射0.05ug白介素1-β(IL-1b)或正畸机械刺激后c-fos样免疫反应(For-IR)在氯醛糖麻醉Wistar大鼠三叉神经尾侧的表达。注射IL-1b诱导的Fos-IR(IL-1b)可在注射后早期给予非类固醇抗炎药(NSAIDs)抑制。结果提示，牙周病变部位少量IL-1b可诱导痛处理神经元Fos-IR。2.观察牙髓刺激后谷氨酸受体拮抗剂和激动剂对Fos-IR表达的影响。分别于刺激前10min(0.2ms、0.6 mA、0.5 Hz、10min)鞘内注射MK-801、CNQX、AP-3、NMDA、AMPA和佛波酯，观察刺激后2 h各药物对尾侧Fos-IR表达的影响。结果表明，钙离子通过NMDA受体的内流对c-Fos的诱导和持续性疼痛的发展至关重要。

项目成果

半場 道子: "歯髄刺激後の脳内c-fos発現におけるglutamate receptorの役割" 歯科基礎医学会雑誌. 36. 165- (1995)

Michiko Hanba：“牙髓刺激后谷氨酸受体在脑 c-fos 表达中的作用”日本基础牙科医学会杂志 36. 165- (1995)。

Hamba,M.: "Loxoproten-Na blocked c-fos immunoreactivity" APLAR Congress of Rheumatology (Asia Pacific Rheumatology Health Association). 8. 2 (1996)

Hamba,M.：“Loxoproten-Na 阻断了 c-fos 免疫反应性”APLAR 风湿病学大会（亚太风湿病健康协会）。

半場 道子: "歯髄刺激後の脳内c-fosにおけるglutamate receptorの役割" 歯科基礎医学会雑誌. 37. 165 (1995)

Michiko Hanba：“牙髓刺激后大脑中 c-fos 中谷氨酸受体的作用”日本基础牙科医学会杂志 37. 165 (1995)。

Hmba, M: "Loxoprofen-Na blocked c-fos immunoreactivity." APLAR Congress of Rheumatology (Asia Pacific Rheumatology Health Association). 8. 2 (1986)

Hmba，M：“洛索洛芬钠阻断了 c-fos 免疫反应性。”

半場 道子: "歯に矯正力を加えた際のc-Fos陽性細胞の脳内分布とNMDA,MK-801,INDOMETHACIN投与の影響" 歯科基礎医学会雑誌. 36. 231 (1994)

Michiko Hanba：“对牙齿施加正畸力时大脑中 c-Fos 阳性细胞的分布以及 NMDA、MK-801 和吲哚美辛的给药效果”日本基础牙科医学会杂志 36. 231。 (1994)