Pathophysiological studies of platelet-associated immunoglobulins.

特发性血小板减少性紫癜(ITP)是由于一种抗血小板相关抗原的抗体引起的，随后网状内皮系统会吞噬血小板。因此，ITP是一种自身免疫性疾病，ITP与系统性红斑狼疮(SLE)的关系备受关注。因此，我们检测了两种疾病患者的血小板相关免疫球蛋白G(PAIg G)、PAIg M、PAIg A和PAC3，并进行了比较。ITP患者的血小板减少和血管内存活时间缩短的程度多数高于SLE患者。而SLE患者PAC3升高的发生率低于ITP患者。在这两种疾病中，抗血小板抗体Ig G和Ig A、Ig M、C3的升高与血小板减少程度的相关性要好于单项抗体的升高，因此，同时检测这些抗体在两种疾病的临床研究中是有价值的。对1986年建立的微量酶联免疫吸附试验系统检测PAIg G进行了研究，建立了可靠的检测方法。因此，我们将这些结果与免疫过氧化物酶方法的结果进行了比较。我们发现，只有当使用相同的酶标记抗体时，这些结果才密切相关。此外，我们试图建立一种简单而敏感的检测方法，用于多次输注血小板的患者，并开发了一种依赖于肉眼的亲和素-生物素-过氧化物酶复合体的简便方法。

Idiopathic thrombocytopenic purpura (ITP) is due to an antibody against a platelet-associated antigen with subsequent platelet phagocytosis by the reticuloendotherial system. Therefore,ITP is one of the autoimmune diseases and the relationship between ITP and systemic lupus erythematosus (SLE) has received much attention. So, we measured the values of platelet-associated IgG (PAIgG), PAIgM , PAIgA and PAC3 in both diseases and compared these values.In the patients of ITP, the degree of thrombocytopenia and shortening of the intravascular survival of platelets is in most cases higher than in those of SLE. While , in the patients of SLE , the fequency of elevated PAC3 is lower than in those of ITP. In the patients of either , elevation of IgG antiplatelet antibodies in combination with IgA , IgM , C3 was better correlated with the level of thrombocytopenia than elevation of only these antibodies.Therefore , it is suggested that the simultaneous measurement of these antibodies is useful in the clinical study of both diseases.While , there are many assays for the measurement of PAIgG , normal values of PAIgG are variable according to the different assays. We investigated the assay of PAIgG by the Micro ELISA system from 1986,and established a reliable method. So , we compared these results with those of the immunoperoxidase method. We found , these results crrelated closely with each other only when the same enzyme labeled antibody was used.Moreover , we attempted to establish a simple and sensitive assay of platelet-bound IgG (PBIgG) for the patients who received many platelet transfusions, and developed a simple method relying on the naked eye using avidin -biotin-peroxidase complex.