Pathophysiological studies of platelet-associated immunoglobulins.

特发性血小板减少性紫癜（ITP）是由于抗血小板相关抗原的抗体引起的，随后血小板被网状内皮系统吞噬。因此，ITP是一种自身免疫性疾病，其与系统性红斑狼疮（SLE）的关系备受关注。因此，我们测定了血小板相关IgG（PAIgG）、PAIgM、PAIgA和PAC_3在两种疾病中的值，并进行了比较。而在SLE患者中，PAC 3升高的频率低于ITP患者。在两种疾病中，IgG抗血小板抗体与伊加、IgM、C_3的联合升高与血小板减少的相关性比单独升高更好，因此，同时测定这些抗体对两种疾病的临床研究是有用的。PAIgG的正常值根据不同的测定而变化。我们从1986年开始研究用微量ELISA系统测定PAIgG，建立了一种可靠的方法。因此，我们将这些结果与免疫过氧化物酶法的结果进行了比较。我们还尝试建立一种简便、灵敏的测定多次血小板输注病人血小板结合IgG（PBIgG）的方法，并建立了一种简便的肉眼检测法，即用亲和素-生物素-过氧化物酶复合物。

Idiopathic thrombocytopenic purpura (ITP) is due to an antibody against a platelet-associated antigen with subsequent platelet phagocytosis by the reticuloendotherial system. Therefore,ITP is one of the autoimmune diseases and the relationship between ITP and systemic lupus erythematosus (SLE) has received much attention. So, we measured the values of platelet-associated IgG (PAIgG), PAIgM , PAIgA and PAC3 in both diseases and compared these values.In the patients of ITP, the degree of thrombocytopenia and shortening of the intravascular survival of platelets is in most cases higher than in those of SLE. While , in the patients of SLE , the fequency of elevated PAC3 is lower than in those of ITP. In the patients of either , elevation of IgG antiplatelet antibodies in combination with IgA , IgM , C3 was better correlated with the level of thrombocytopenia than elevation of only these antibodies.Therefore , it is suggested that the simultaneous measurement of these antibodies is useful in the clinical study of both diseases.While , there are many assays for the measurement of PAIgG , normal values of PAIgG are variable according to the different assays. We investigated the assay of PAIgG by the Micro ELISA system from 1986,and established a reliable method. So , we compared these results with those of the immunoperoxidase method. We found , these results crrelated closely with each other only when the same enzyme labeled antibody was used.Moreover , we attempted to establish a simple and sensitive assay of platelet-bound IgG (PBIgG) for the patients who received many platelet transfusions, and developed a simple method relying on the naked eye using avidin -biotin-peroxidase complex.