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Species differences in platelet function

血小板功能的物种差异

基本信息

批准号：
61580043
负责人：
TANIMOTO Yoshifumi
金额：
$ 1.41万
依托单位：
Central Institute for Experimental Animals
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1986
资助国家：
日本
起止时间：
1986 至 1988
项目状态：
已结题

项目摘要

The calcium ion sensitive photo-protein aequorin was introduced by incubation into washed platelets of human, monkey, dog, rabbit, and rat. The intracellular calcium ion concentration in mediating thrombin-induced platelet activation differed in value among animals but the phases of efflux were almost the same. Two phases of Ca ion efflux in collagen-induced activation of human, monkey, and rat platelets became one phase upon addition of indomethacin. These findings indicated differences in the values and the phase of intracellular calcium ion efflux with each stimulation in animals.Human, rhesus and cynomolgus monkey blood platelet ATP contents were determined using a commercial RIA kit. The results showed the relation human > rhesus > cynomolgys. The concentration of human platelet factor 4 (PF4) showed a value 5.2-fold that of rabbit, and a value 8.1-8.2-fold that of other animals. These findings indicated differences in the storage pool in the platelets.The proteins and glycoproteins of the human and monkey membranes as determined SDS-polyacrylamide gel electrophoresis showed mutually similar patterns, but were clearly different from those of the other animals.Platelet retention by glass beads was higher in rat than human and decreased with aging in contrast with the case in human.In order to measure the cyclo-oxygenase and lipoxygenase activites in rat platelets, it was necessary to increase the arachidonic acid concentration as substrate. The enzyme activites in the rat were found to be much lower than in human.Difference in the ATP release pattern in ADP stimulated platelets of rabbit and dog suggested the existance of differences in the number and localization of platelet dense granules according to electron microscopic observations.These findings show the necessity of further investigation of species differences in platelet functions.

通过孵育将钙离子敏感性光蛋白水母发光蛋白引入人、猴、狗、兔和大鼠的洗涤血小板中。在介导凝血酶诱导的血小板活化的细胞内钙离子浓度在动物之间的值不同，但流出的阶段几乎是相同的。在胶原诱导的人、猴和大鼠血小板活化中，钙离子流出的两个阶段在加入吲哚美辛后变为一个阶段。这些结果表明，在不同的值和时相的细胞内钙离子流出与每一个stimulations.Human，恒河猴和食蟹猴血小板ATP含量测定使用商业RIA试剂盒。结果表明，人>恒河猴>食蟹猴。人血小板因子4（PF 4）的浓度显示为兔的5.2倍，为其他动物的8.1-8.2倍。这些发现表明血小板储存池的差异。SDS-聚丙烯酰胺凝胶电泳测定的人和猴血小板膜的蛋白质和糖蛋白显示出相互相似的模式，玻璃珠法测定的大鼠血小板滞留率高于人，并随年龄增长而降低，与人相比，结果表明，花生四烯酸是诱导大鼠血小板氧合酶和脂氧合酶活性的重要底物，增加花生四烯酸的浓度是必要的。结果表明，大鼠血小板ATP酶活性明显低于人，ADP刺激的家兔和狗血小板ATP释放模式的差异，提示血小板致密颗粒的数量和分布存在差异，提示有必要进一步研究血小板功能的种属差异。