Development of Immunological Reagents for the Identification of New World Monkey Biomarkers

开发用于鉴定新世界猴生物标志物的免疫试剂

• 批准号: 10807651
• 负责人: Luis David Giavedoni
• 金额: $ 15.53万
• 依托单位: TRINITY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-01 至 2026-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10807651
• 关键词: Administrative Supplement; Aging; Animal Model; Animals; Antibodies; Archives; Award; Biological; Biological Assay; Biological Markers; Biology; Blood specimen; Breeding; C-reactive protein; CXCL10 gene; CXCR3 gene; Callithrix; Cebidae; Cells; Chronic Disease; Collaborations; Communicable Diseases; Communities; Data; Development; Enrollment; Enzyme-Linked Immunosorbent Assay; Epitope Mapping; Epitopes; Equipment; Eukaryotic Cell; Flow Cytometry; Fluorescent Dyes; Funding; Generations; Goals; Granzyme; Hormones; Human Resources; Immune; Immunobiology; Immunologic Techniques; Immunologics; Immunology procedure; Immunophenotyping; Inflammation; Inflammatory; Institution; Insulin; Interferon Type II; Interleukin-10; Interleukin-4; Interleukin-6; Laboratories; Lasers; Learning; Leptin; Maintenance; Membrane Glycoproteins; Metabolic; Metabolic hormone; Metabolic syndrome; Metabolism; Modeling; Modernization; Monkeys; Monoclonal Antibodies; Multiple Sclerosis; Mus; Neurosciences; Night Monkey; Obesity; Output; Parents; Pathology; Pre-Clinical Model; Procedures; Process; Production; Proteins; Protocols documentation; R24; Reagent; Recombinant Proteins; Recombinants; Reproducibility; Reproduction; Research; Research Personnel; Resources; Saimiri; Sampling; Science; South American; Stains; Students; Techniques; Testing; Texas; Tissue Sample; Training; Transfection; Tube; United States National Institutes of Health; Universities; Validation; Western Blotting; access restrictions; adiponectin; age related neurodegeneration; assay development; biomarker identification; cross reactivity; cytokine; detector; enzyme linked immunospot assay; experience; human disease; human model; improved; innovation; instrument; instrumentation; interest; member; murine monoclonal antibody; new technology; novel; programs; tool; training opportunity; undergraduate student; university student; validation studies; virology

PROJECT SUMMARY This R24 OD030215 Administrative Supplement request seeks funds for the acquisition of a Cytek Northern Lights spectral flow cytometer. The requested instrument is equipped with three excitation laser lines, 16 detectors per laser, an automatic sample loader, and basic start-up Training Kit; the instrument will be housed in the Center for Sciences and Innovation (CSI) building of Trinity University in San Antonio, Texas. The goal of the “New World Monkey Immunoreagent Resource” parent award is to improve the translational value of the New World Monkey (NWM) species marmoset (MAR), squirrel monkey (SQM), and owl monkey (OLM) by generating novel monoclonal antibodies and assays able to identify important biomarkers of inflammation and metabolism, for which there is scarcity of reagents. The need for this instrument is based on the following reasons: 1) It will significantly improve the development of the immunological assays proposed in Specific Aim 2 of the parent award; these flow cytometry assays apply to six of the ten selected targets (IL-4, IL-6, IL-10, IP-10, granzyme B, and CD69) and will expand the limited assay scope caused by the use of antiquated instrumentation. 2) It will increase the rigor and reproducibility of these assays by concentrating assay development for all three NWM species in one laboratory; administrative changes in one of our collaborating institutions reduces access to more modern instruments and limits assays to just one species. 3) It will generate deeper scientific output from the limited number of cells that can be recovered from these small monkeys; spectral flow cytometry allows the use of common fluorescent dyes that are incompatible in conventional flow cytometry and can use archived spectral data, which reduces the number of cells used for control tubes. 4) It will generate new avenues for training undergraduate students and technical personnel in the practical understanding of spectral flow cytometry, a technique that has revolutionized immunophenotyping; presence of this instrument at Trinity University will give access to novel technologies to upper division Biology students. The capacity of the spectral flow cytometer to detect a large number of markers in a single cell will increase the ability to identify multiple cell subsets in small blood or tissue samples, which is critical for improving the translational value of these small body NWM species. Institutional support is provided for the long-term maintenance of this equipment.

项目概要 此 R24 OD030215 行政补充请求寻求资金用于收购 Cytek Northern Lights 光谱流式细胞仪。所要求的仪器配备了三个 激发激光线、每个激光器 16 个探测器、自动样品加载器和基本启动 训练套件；该仪器将安置在科学与创新中心 (CSI) 位于德克萨斯州圣安东尼奥市的三一大学大楼。 “新世界猴子”的目标 免疫试剂资源”母奖是为了提高新产品的转化价值 世界猴 (NWM) 物种狨猴 (MAR)、松鼠猴 (SQM) 和猫头鹰猴 （OLM）通过生成新型单克隆抗体和能够识别重要的检测方法 炎症和代谢的生物标志物，目前缺乏试剂。需要 选用该仪器基于以下原因： 1）它将显着提高 开发家长奖具体目标 2 中提出的免疫检测方法； 这些流式细胞术检测适用于十个选定靶标中的六个（IL-4、IL-6、IL-10、IP-10、 颗粒酶 B 和 CD69），并将扩大由于使用而造成的有限测定范围 陈旧的仪器。 2) 它将通过以下方式提高这些测定的严谨性和再现性 在一个实验室集中开发所有三种 NWM 物种的检测方法；行政 我们合作机构之一的变化减少了获得更现代工具的机会， 将测定仅限于一种物种。 3）它将从有限的资源中产生更深入的科学成果 可以从这些小猴子身上回收的细胞数量；光谱流式细胞术允许 使用与传统流式细胞术不兼容的常见荧光染料， 可以使用存档的光谱数据，这减少了用于控制管的单元数量。 4）它 将为培养本科生和技术人员开辟新的途径 对光谱流式细胞术的实际理解，这项技术彻底改变了 免疫表型分析；三一大学的这一仪器的存在将提供新颖的 向高年级生物学学生提供技术。光谱流式细胞仪的容量 检测单个细胞中的大量标记将增加识别多个细胞的能力 小血液或组织样本中的子集，这对于提高转化价值至关重要 这些体型较小的 NWM 物种。为长期维护提供制度支持 该设备的。