Development of Immunological Reagents for the Identification of New World Monkey Biomarkers

开发用于鉴定新世界猴生物标志物的免疫试剂

• 批准号: 10807651
• 负责人: Luis David Giavedoni
• 金额: $ 15.53万
• 依托单位: TRINITY UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2023
• 资助国家: 美国
• 起止时间: 2023-06-01 至 2026-01-31
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/10807651
• 关键词: Administrative Supplement; Aging; Animal Model; Animals; Antibodies; Archives; Award; Biological; Biological Assay; Biological Markers; Biology; Blood specimen; Breeding; C-reactive protein; CXCL10 gene; CXCR3 gene; Callithrix; Cebidae; Cells; Chronic Disease; Collaborations; Communicable Diseases; Communities; Data; Development; Enrollment; Enzyme-Linked Immunosorbent Assay; Epitope Mapping; Epitopes; Equipment; Eukaryotic Cell; Flow Cytometry; Fluorescent Dyes; Funding; Generations; Goals; Granzyme; Hormones; Human Resources; Immune; Immunobiology; Immunologic Techniques; Immunologics; Immunology procedure; Immunophenotyping; Inflammation; Inflammatory; Institution; Insulin; Interferon Type II; Interleukin-10; Interleukin-4; Interleukin-6; Laboratories; Lasers; Learning; Leptin; Maintenance; Membrane Glycoproteins; Metabolic; Metabolic hormone; Metabolic syndrome; Metabolism; Modeling; Modernization; Monkeys; Monoclonal Antibodies; Multiple Sclerosis; Mus; Neurosciences; Night Monkey; Obesity; Output; Parents; Pathology; Pre-Clinical Model; Procedures; Process; Production; Proteins; Protocols documentation; R24; Reagent; Recombinant Proteins; Recombinants; Reproducibility; Reproduction; Research; Research Personnel; Resources; Saimiri; Sampling; Science; South American; Stains; Students; Techniques; Testing; Texas; Tissue Sample; Training; Transfection; Tube; United States National Institutes of Health; Universities; Validation; Western Blotting; access restrictions; adiponectin; age related neurodegeneration; assay development; biomarker identification; cross reactivity; cytokine; detector; enzyme linked immunospot assay; experience; human disease; human model; improved; innovation; instrument; instrumentation; interest; member; murine monoclonal antibody; new technology; novel; programs; tool; training opportunity; undergraduate student; university student; validation studies; virology

PROJECT SUMMARY This R24 OD030215 Administrative Supplement request seeks funds for the acquisition of a Cytek Northern Lights spectral flow cytometer. The requested instrument is equipped with three excitation laser lines, 16 detectors per laser, an automatic sample loader, and basic start-up Training Kit; the instrument will be housed in the Center for Sciences and Innovation (CSI) building of Trinity University in San Antonio, Texas. The goal of the “New World Monkey Immunoreagent Resource” parent award is to improve the translational value of the New World Monkey (NWM) species marmoset (MAR), squirrel monkey (SQM), and owl monkey (OLM) by generating novel monoclonal antibodies and assays able to identify important biomarkers of inflammation and metabolism, for which there is scarcity of reagents. The need for this instrument is based on the following reasons: 1) It will significantly improve the development of the immunological assays proposed in Specific Aim 2 of the parent award; these flow cytometry assays apply to six of the ten selected targets (IL-4, IL-6, IL-10, IP-10, granzyme B, and CD69) and will expand the limited assay scope caused by the use of antiquated instrumentation. 2) It will increase the rigor and reproducibility of these assays by concentrating assay development for all three NWM species in one laboratory; administrative changes in one of our collaborating institutions reduces access to more modern instruments and limits assays to just one species. 3) It will generate deeper scientific output from the limited number of cells that can be recovered from these small monkeys; spectral flow cytometry allows the use of common fluorescent dyes that are incompatible in conventional flow cytometry and can use archived spectral data, which reduces the number of cells used for control tubes. 4) It will generate new avenues for training undergraduate students and technical personnel in the practical understanding of spectral flow cytometry, a technique that has revolutionized immunophenotyping; presence of this instrument at Trinity University will give access to novel technologies to upper division Biology students. The capacity of the spectral flow cytometer to detect a large number of markers in a single cell will increase the ability to identify multiple cell subsets in small blood or tissue samples, which is critical for improving the translational value of these small body NWM species. Institutional support is provided for the long-term maintenance of this equipment.

项目总结 此R24 OD030215行政补充申请寻求资金，用于收购 Cytek Northern Lights光谱流式细胞仪。所请求的仪器配备了三个 激励激光线路，每个激光器16个探测器，一个自动样品加载器，以及基本启动 培训套件；该仪器将放置在科学与创新中心(CSI) 德克萨斯州圣安东尼奥的三一大学大楼。《新世界猴子》的目标 《免疫制剂资源》家长奖是提高翻译价值的新方法 世界猕猴(NWM)物种绒猴(MAR)、松鼠猴(SQM)和猫头猴 (OLM)通过产生新的单抗和能够识别重要的 炎症和新陈代谢的生物标志物，缺乏试剂。需要 因为这个工具是基于以下原因的：1)它将显著提高 发展父母奖特定目标2中提出的免疫学检测方法； 这些流式细胞术检测适用于10个选定靶点中的6个(IL-4、IL-6、IL-10、IP-10、 颗粒酶B和CD69)，并将扩大由于使用 陈旧的仪器。2)它将通过以下方式提高这些检测的严密性和重复性 在一个实验室集中开发所有三种NWM的分析方法；行政管理 我们的一个合作机构的变化减少了获得更现代工具的机会， 将化验限制为只有一个物种。3)它将从有限的 可以从这些小猴子身上回收的细胞数量；光谱流式细胞术允许 使用常规流式细胞术中不相容的常见荧光染料和 可以使用存档的光谱数据，这减少了用于控制管的细胞数量。4)它 将为培养本科生和技术人员创造新的途径 对光谱流式细胞术的实际理解，这是一项革命性的技术 免疫表型；这种仪器在三一大学的出现将使人们能够获得新的 向高年级生物专业学生传授技术。光谱流式细胞仪的能力 检测单个细胞中的大量标记将提高识别多个细胞的能力 小的血液或组织样本中的亚群，这对于提高 这些体型较小的NWM物种。为长期维护提供制度支持 这台设备的。