Transport of Phosphoenolpyruvate across the Biological Membrane

磷酸烯醇丙酮酸跨生物膜的运输

• 批准号: 62045042
• 负责人: HAMASAKI Naotaka
• 金额: $ 2.75万
• 依托单位: Fukuoka University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Overseas Scientific Survey.
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62045042/
• 关键词: Phosphoenolpyruvate transport across the cell membrane; Red Cell Membrane; Band 3; Phosphate transport system; Histidine residue; Mediated transport; Structure of transmembrane protein

This project is concerned with the transport mechanisms of phosphoenolpyruvate across the red cell membrane. Results of this project are summarized as follows: 1. Transport of phosphoenolpyruvate is mediated by Band 3 protein of red cell membrane as well as the transports of chloride and phosphate. 2. The 60th-lysine residue from the COOH-terminal end of Band 3 constitutes at least a part of the active center of the transport system. 3. In addition to the lysine residue, a histidine residue within Band 3 participates in the transport system. The histidine is located at the intracellular surface of the cell membrane and allosterically interacts with the transfer site. 4. Phosphoenolpyruvate transports across not only the red cell membrane but also the cell membranes of kidney and liver.

本项目研究的是磷酸烯醇式丙酮酸在红细胞膜上的转运机制。本课题的研究结果总结如下：1.红细胞膜上的带3蛋白以及氯离子和磷酸盐的转运都参与了磷烯醇式丙酮酸的转运。2.带3的COOH末端的60位赖氨酸残基至少构成运输系统活性中心的一部分。3.除了赖氨酸残基外，带3内的组氨酸残基还参与运输系统。组氨酸位于细胞膜的胞内表面，并与转移部位变构相互作用。4.磷酸烯醇式丙酮酸不仅可跨红细胞膜转运，还可跨肾、肝细胞膜转运。

项目成果

Naotaka Hamasaki: "Anion Transport Center of Band3 in Erythrocyte Membranes" Studia Biophysica. 134. 127-131 (1989)

Naotaka Hamasaki：“红细胞膜中 Band3 的阴离子转运中心”Studia Biophysicala。

J.Biochem.100巻,1号. (1986)

J.Biochem.第 100 卷，第 1 期。(1986)

Naotaka Hamasaki: "Anion Transport Protein of the Red Blood Cell Membrane" Elsevier Science Publishers, 230 (1989)

Naotaka Hamasaki：“红细胞膜的阴离子转运蛋白”Elsevier Science Publishers，230 (1989)

Trends in Biochem.Sci.(TIBS). 12巻,5号. (1987)

生物化学趋势 (TIBS)，第 12 卷，第 5 期。(1987)

Kenji Izuhara: "Conformational Change of Band3 Protein Induced by Diethylpyrocarbonate Modification in Human Erythrocyte Ghosts" Biochemistry. 28. 4725-4728 (1989)

Kenji Izuhara：“人红细胞鬼影中焦碳酸二乙酯修饰诱导的 Band3 蛋白构象变化”生物化学。