Transport of Phosphoenolpyruvate across the Biological Membrane

本项目关注磷酸烯醇丙酮酸在红细胞膜上的转运机制。本课题的研究成果总结如下：1。磷酸烯醇丙酮酸的转运是由红细胞膜上的Band 3蛋白介导的，同时还包括氯离子和磷酸盐的转运。2. 第60个赖氨酸残基从带3的cooh末端至少构成了运输系统的活性中心的一部分。3. 除了赖氨酸残基外，Band 3内的组氨酸残基也参与了运输系统。组氨酸位于细胞膜的胞内表面，并与转移位点发生变构相互作用。4. 磷酸烯醇丙酮酸不仅在红细胞膜上转运，而且在肾和肝细胞膜上转运。

This project is concerned with the transport mechanisms of phosphoenolpyruvate across the red cell membrane. Results of this project are summarized as follows: 1. Transport of phosphoenolpyruvate is mediated by Band 3 protein of red cell membrane as well as the transports of chloride and phosphate. 2. The 60th-lysine residue from the COOH-terminal end of Band 3 constitutes at least a part of the active center of the transport system. 3. In addition to the lysine residue, a histidine residue within Band 3 participates in the transport system. The histidine is located at the intracellular surface of the cell membrane and allosterically interacts with the transfer site. 4. Phosphoenolpyruvate transports across not only the red cell membrane but also the cell membranes of kidney and liver.