Structural Study on Human Red Cell Band 3 Protein, AE1.

人红细胞带 3 蛋白 AE1 的结构研究。

• 批准号: 21590322
• 负责人: HAMASAKI Naotaka
• 金额: $ 2.91万
• 依托单位: Nagasaki International University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 2009
• 资助国家: 日本
• 起止时间: 2009 至 2011
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-21590322/
• 关键词: 生体分子医学; バンド3タンパク質; 膜貫通領域; 膜タンパク質; 結晶化; X線構造解析; 電子線構造解析; AE1; 陰イオン透過

The membrane domain of human erythrocyte anion exchanger 1(AE1) works as a Cl^-/HCO_3^-antiporter. This exchange is a key step for CO_2/O_2 circulation in the blood. In spite of their importance, structural information about AE1 and the AE(Anion Exchanger) family is still very limited. We used the electron microscopy and the X-ray diffraction methods to solve the three-dimensional structure of the AE1 membrane domain, fixed in an outward-open conformation by cross-linking. A dimer of AE1 membrane domains packed in two-dimensional array showed a projection map similar to that of the prokaryotic homolog of ClC chloride channel, a Cl^-/H^+antiporter. In a three-dimensional map, there are V-shaped densities near the center of the dimer, and slightly narrower V-shaped clusters at a greater distance from the center of the dimer. These appear to be inserted into membrane from opposite sides. The structural motifs, two homologous pairs of helices in internal repeats of ClC transporter(helices B+C and J+K), are well fitted to those AE1 densities after simple domain movement. We could get good enough results with a high resolution for examining the structure and function relationship of the anion exchange in red blood cells.

人红细胞阴离子交换蛋白1（AE 1）膜结构域是Cl^-/HCO_3^-逆向转运蛋白。这种交换是血液中CO_2/O_2循环的关键步骤。尽管它们的重要性，关于AE 1和AE（阴离子交换剂）家族的结构信息仍然非常有限。我们使用电子显微镜和X-射线衍射的方法来解决的三维结构的AE 1膜域，固定在一个向外开放的构象通过交联。AE 1膜结构域的二聚体排列成二维阵列，其投影图与ClC氯离子通道（一种Cl^-/H^+反向转运蛋白）的原核同源物相似。在三维图中，二聚体中心附近有V形密度，距离二聚体中心较远的地方有稍窄的V形簇。它们似乎从相反的两侧插入膜中。C1 C转运蛋白内部重复序列中的两对同源螺旋（螺旋B+C和J+K）结构基序与简单结构域移动后的AE 1密度非常吻合。在高分辨率条件下，可以得到足够好的结果，用于研究红细胞阴离子交换的结构与功能关系。

项目成果

抗ヒトバンド3モノクローナル抗体

抗人带3单克隆抗体

• 发表时间: 2011

Liquid Phase Immunoassays utilizing magnetic marker and SQUID magneto-meter

利用磁性标记和 SQUID 磁力计的液相免疫测定

• 发表时间: 2010
• 期刊: Clin.Chem.Lab.Med. 48(9)
• 作者: H.Kuma;H.Oyamada;A.Tsukamoto;T.Mizoguchi;A.Kandori;Y.Sugiura;K.Yoshinaga;K.Enpuku;N.Hamasaki
• 通讯作者: N.Hamasaki

Mutation of His 834 in human anion exchange 1 affects substrate binding

人阴离子交换 1 中 His 834 的突变影响底物结合

• 发表时间: 2010
• 期刊: Biochem. Biophy. Acta
• 作者: Takazaki;S;Abe;Y;Yamaguchi T;Yagi M;Ueda T;Kagn D;and Hamasaki N.
• 通讯作者: and Hamasaki N.

Mutation of His 834 in human anion exchanger 1 affects substrate binding.

• DOI: 10.1016/j.bbamem.2010.01.019
• 发表时间: 2010-05
• 期刊: Biochimica et biophysica acta
• 作者: Shinya Takazaki;Y. Abe;Tomohiro Yamaguchi;Mikako Yagi;T. Ueda;D. Kang;N. Hamasaki

Helical image reconstruction of the outward-open human erythrocyte band 3 membrane domain in tubular crystals.

• DOI: 10.1016/j.jsb.2009.12.009
• 发表时间: 2010-03
• 期刊: Journal of structural biology
• 影响因子: 3
• 作者: Tomohiro Yamaguchi;T. Fujii;Y. Abe;T. Hirai;D. Kang;K. Namba;N. Hamasaki;K. Mitsuoka