Development of a new assay system for protein S/protein C and treatment of thrombophilia.

开发新的蛋白 S/蛋白 C 检测系统和血栓形成倾向的治疗。

• 批准号: 16390165
• 负责人: HAMASAKI Naotaka
• 金额: $ 9.15万
• 依托单位: KYUSHU UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (B)
• 财政年份: 2004
• 资助国家: 日本
• 起止时间: 2004 至 2005
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-16390165/
• 关键词: Deep vein thrombosis; protein S gene mutation; protein C gene mutation; factor V Leiden; Javanese thrombophilia; protein S_<Tokushima>; 血栓性素因; 血栓症; プロテインC; プロテインS; 分子異常性; 血栓症治療薬

Coagulation factor V Leiden has not been detected in Japanese patients suffering from thromboses. We have performed a systematic investigation to find out genetic risk factors for thromboses in Japanese population. Our study indicates that the frequency (19/85=0.22) of mutations of protein S gene in the Japanese patients was 5-10 times higher than that of mutations of protein S gene in Caucasian patients, and the frequency (8/85=0.09) of mutations of protein C gene was almost three times higher than that of Caucasian patients, suggesting that Protein S/Protein C anomaly is the major risk factor for Japanese thrombophilia. During this study, we established a new quantitative method for Protein S/Protein C function. In addition, we found special components, which activate Protein S/Protein C activities. Now, we have tried to apply these components for the treatment of thromboses.

日本血栓患者体内未检测到凝血因子V莱顿。我们进行了一项系统的调查，以找出日本人群血栓形成的遗传危险因素。我们的研究表明，日本人S蛋白基因突变频率(19/85=0.22)是高加索人S蛋白基因突变频率的5~10倍，蛋白C基因突变频率(8/85=0.09)几乎是高加索人的3倍，提示蛋白S/蛋白C异常是日本人血栓形成的主要危险因素。在本研究中，我们建立了一种新的定量检测蛋白S/蛋白C功能的方法。此外，我们还发现了激活蛋白S/蛋白C活性的特殊成分。现在，我们已经尝试将这些成分应用于血栓的治疗。

项目成果

Analytical goals for coagulation tests based on biological variation

• DOI: 10.1515/cclm.2004.015
• 发表时间: 2004-02
• 期刊: Clinical Chemistry and Laboratory Medicine (CCLM)
• 作者: Y. Wada;M. Kurihara;M. Toyofuku;M. Kawamura;H. Iida;Y. Kayamori;S. Kinoshita;N. Hamasaki

Characterization of two novel mutations of the antithrombin gene observed in Japanese thrombophilic patients.

• DOI: 10.1016/j.thromres.2004.08.025
• 发表时间: 2005
• 期刊: Thrombosis research
• 影响因子: 7.5
• 作者: M. Kurihara;Kumiko Watanabe;S. Inoue;Y. Wada;Miyuki Ono;M. Wakiyama;H. Iida;S. Kinoshita;N. Hamasaki

Protein S and protein C gene mutations in Japanese deep vein thrombosis patients

• DOI: 10.1016/j.clinbiochem.2005.05.006
• 发表时间: 2005-10-01
• 期刊: CLINICAL BIOCHEMISTRY
• 影响因子: 2.8
• 作者: Kinoshita, S;Iida, H;Hamasaki, N
• 通讯作者: Hamasaki, N

Successful therapy with argatroban for superior mesenteric vein thrombosis in a patient with congenital antithrombin deficiency.

阿加曲班成功治疗先天性抗凝血酶缺乏症患者的肠系膜上静脉血栓形成。

• 发表时间: 2005
• 期刊: Eur J Haematol 75(2)
• 作者: Muta;T.;et al.
• 通讯作者: et al.

Development of a new colorimetric method for protein S activity measurement.

开发一种用于蛋白质 S 活性测量的新比色方法。

• 发表时间: 2004
• 期刊: Clin Chem Lab Med 42(3)
• 作者: Tsuda;T.;et al.
• 通讯作者: et al.