Basic Research on the Development of Recombinant Vaccines for Animals by Genetic Engineering

该研究项目的目的是为兽医开发重组疫苗提供基础数据。重组疫苗由载体病毒和抗原基因两部分组成。在本研究中，我们使用了两种可用于重组疫苗载体的病毒。一种是用于马立克氏病疫苗的火鸡疱疹病毒，另一种是禽痘病毒。我们确定胸苷激酶基因作为载体病毒基因组与抗原基因之间基因重组的标记。将新城疫病毒血凝素-神经氨酸酶基因克隆到鸡痘病毒胸苷激酶位点。在流感病毒和新城疫病毒的许多毒株中观察到抗原基因的突变，作为抗原选择的基础资料。如果能大量获得抗原基因产物，可作为组份疫苗。本课题利用杆状病毒载体获得抗原产物。利用该载体可获得马立克病病毒单纯疱疹病毒gB样蛋白、新城疫病毒血凝素-神经氨酸酶抗原和马立克病病毒gA。这些蛋白具有生物活性，可用作成分疫苗。通过本课题的研究，为构建可用于兽医实践的重组疫苗提供了基础资料。

The aim of this research project is to get basic data to develop recombinant vaccines for veterinarians. Recombinant vaccines consisted of two components, vector virus and antigen genes. In this study, we used two viruses which can be used for vector of recombinant vaccines. One is herpesvirus of turkeys which is used as a vaccine of Marek's disease virus and the other is fowl pox virus. We decided thymidine kinase gene as a marker for genetic recombination between the vector virus gnome and antigen gene. And hemagglutinin-neuraminidase gene of Newcastle disease virus was cloned into thymidine kinase site of fowl pox virus. Mutations of the antigen genes of influenza virus and Newcastle disease virus were observed in many strains of these viruses as the basic data for selection of antigens.Antigenic gene products can be used as component vaccine, if one can get them with large quantity. In this research project, antigenic products were obtained using baculovirus vector. Using this vector we could get herpes simplex virus gB like protein of Marek's disease virus, hemagglutinin-neuraminidase antigen of Newcastle disease virus and gA of Marek's disease virus. These proteins were biologically active and may be used as component vaccines.In this research project, we could get many basic data to construct the recombinant vaccines which can be used for veterinary practice.