Exploration of the new components for elastic fiber assembly

弹性纤维组装新组件的探索

• 批准号: 24791181
• 负责人: SATO Fumiaki
• 金额: $ 2.66万
• 依托单位: Hoshi University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Young Scientists (B)
• 财政年份: 2012
• 资助国家: 日本
• 起止时间: 2012-04-01 至 2014-03-31
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-24791181/
• 关键词: 光老化; 日光弾性線維症; 弾性線維; リジルオキシダーゼ

To investigate the new components for elastic fiber assembly, we focused on the solar elastosis, of which characterization is observed the abnormal elastic fiber in adult dermis. First, we assess the mRNA expression between the dermal fibroblast from neonatal and adult after UV radiation. Because RT-qPCR showed the difference in mRNA expression which related to lysyl oxidase, we clarified the function of lysyl oxidase in elastogenesis. Lysyl oxidase is known to cross-linker between tropoelastin or collagen molecules, resulting in maturatation of elastic fibers or collagen fibers. We found that lysyl oxidase affects the deposition of tropoelastin onto microfibril fibers and localizes on the cell surface in early stage of elastic fiber assembly. Further, the release of lysyl oxidase form the cell surface was delayed in adult dermal fibroblast, resulting in abnormal assembly of elastic fiber. These data suggests that the release of lysyl oxidase is key step of elastogenesis.

为了探索弹性纤维组装的新成分，我们将重点放在日光性弹性组织变性上，其特征是观察到成人真皮中的异常弹性纤维。首先，我们评估了新生儿和成人皮肤成纤维细胞在紫外线照射后的mRNA表达。由于RT-qPCR显示赖氨酰氧化酶相关mRNA表达的差异，我们阐明了赖氨酰氧化酶在弹性蛋白生成中的作用。已知赖氨酰氧化酶在原弹性蛋白或胶原分子之间交联，导致弹性纤维或胶原纤维的成熟。我们发现赖氨酰氧化酶影响原弹性蛋白在微纤维上的沉积，并在弹性纤维组装的早期定位于细胞表面。此外，赖氨酰氧化酶从细胞表面的释放在成人真皮成纤维细胞中延迟，导致弹性纤维的异常组装。这些数据表明赖氨酰氧化酶的释放是弹性蛋白生成的关键步骤。

项目成果

5-aza-2′-deoxycytidine, a DNA methyltransferase inhibitor, facilitates inorganic phosphorus-induced mineralization of vascular smooth muscle cells

5-aza-2-deoxycytidine 是一种 DNA 甲基转移酶抑制剂，可促进无机磷诱导的血管平滑肌细胞矿化

• 期刊: J Atheroscler Thromb
• 影响因子: 4.4
• 作者: Azechi T;Sato F;Sudo R;Wachi H
• 通讯作者: Wachi H

Domain 36 of tropoelastin in elastic fiber formation.

弹性纤维形成中弹性蛋白原的结构域 36。

• 发表时间: 2014
• 期刊: Biological and Pharmaceutical Bulletin
• 影响因子: 2
• 作者: Risa Nonaka;Fumiaki Sato;H. Wachi
• 通讯作者: H. Wachi

Lysyl oxidase enhances the deposition of tropoelastin onto microfibril fibers

赖氨酰氧化酶增强原弹性蛋白在微原纤维上的沉积

• 发表时间: 2013
• 作者: Sato F.;Okada M. and Wachi H
• 通讯作者: Okada M. and Wachi H

ATP13A2 deficiency induces a decrease in cathepsin D activity, fingerprint-like inclusion body formation, and selective degeneration of dopaminergic neurons

• DOI: 10.1016/j.febslet.2013.02.046
• 发表时间: 2013-05-02
• 期刊: FEBS LETTERS
• 影响因子: 3.5
• 作者: Matsui, Hideaki;Sato, Fumiaki;Takahashi, Ryosuke
• 通讯作者: Takahashi, Ryosuke

Trichostatin A, an HDAC Class I/II, Inhibitor, Promotes Pi-Induced Vascular Calcification Via Up-Regulation of the Expression of Alkaline Phosphatase

• DOI: 10.5551/jat.15826
• 发表时间: 2013-01-01
• 期刊: JOURNAL OF ATHEROSCLEROSIS AND THROMBOSIS
• 影响因子: 4.4
• 作者: Azechi, Takuya;Kanehira, Dan;Wachi, Hiroshi
• 通讯作者: Wachi, Hiroshi