Mechanism of Occurrence of Carbohydrate Metabolizing Enzymes in Corn Seeds.

1.一个现象-淀粉酶可能存在于未成熟的玉米种子中，已被观察到。However，这一现象似乎依赖于其他不同类型的碳酸氢盐代谢酶的协同作用，例如，-葡萄糖苷酶、Isoamylase、Q-enzyme、D-enzyme等。这被确认为，你没有-淀粉酶在非活性玉米种子中，淀粉酶在凝胶阶段首次出现，如其他半胱氨酸种子。Fouk kinds of-amylases appeared in the germinated seeds of yellow dent corn。Those enzymes increased rapidly after 4 days of imbibition （27 C， in the dark）.3。淀粉酶II是由各种色谱法的不同类型的纯化的。分子重量约为48，000，最佳pH值为4.7。麦芽糖主要是由solubstarch.4制成的最终产品。麦芽糖的模拟物被证实是由GLC.5制成的。- 淀粉酶II已经被转化为典型的内型酶，从蓝色值的曲线中转化为溶解淀粉的羟基学位。不同葡萄糖降解后遵循的顺序的羟基比率：淀粉样>溶解淀粉淀粉样蛋白>-限制dextrin >-glycogen.7。淀粉酶II在一系列Maltooligoscharides（G_3G_7，G_）中的作用模式<18>由TLC和GLC进行了测试。和Maltooligosaccharides的水合物的速率参数（K_m和K_o）已确定。基于操作模式和速率参数的基础，每个寡藻化合物的splittpoint和活跃站点中的子站点结构已经提前，以及子站点相关性（A_i）已经得到了评价。subsites was assumed to be 7： A_1 = 0.3，A_2=0.3，A_3=0.7， A_4=2.7，A_5+A_6=-2.8，A_7=2.0 （Kcal/mol）。该催化站点估计存在于第5和第6分站点之间。

1. A phenomenon that -amylase may be present in ungerminated corn seeds was observed. However, the phenomenon was presumed to be dependent on the cooperative actions by other different types of carbohydrate metabolizing enzymes, for example, -glucosidase, isoamylase, Q-enzyme, D-enzyme, etc. It was confirmed that thee was no -amylase in ungerminated corn seeds, and -amylase occurred first in the germination stage, like other cereal seeds.2. Fouk kinds of -amylases appeared in the germinated seeds of yellow dent corn. Those enzymes increased rapidly after 4 days of imbibition (27゜C, in the dark).3. An -amylase II was highly purified by various kinds of chromatographies. The molecular weight was about 48,000 and the optimum pH, 4.7. Maltose was mainly formed as final product from soluble starch.4. Anomer of maltose produced was confirmed to be -form by GLC.5. -amylase II was turned out to be typical endo-type enzyme from the curve of blue value for the hydrolysis degree of soluble starch.6. The rates of hydrolyses for vaious -glucans decreased the following orde: amylose > soluble starch amylopectin > -limit dextrin >> glycogen.7. Action patterns of -amylase II on a series of maltooligosaccharides (G_3 G_7, G_<18>) were examined by TLC and GLC. And the rate parameters (K_m and K_o) for the hydrolyese of maltooligosaccharides were determined.8. On the basis of the action patterns and the rate parameters, the splitting point of each oligosaccharides and the subsite structure in active site were presumed, and the subsite affinities (A_i) were evalutaed. The number of subsites was assumed to be 7: A_1 = 0.3,A_2=0.3,A_3=0.7, A_4=2.7,A_5+A_6=-2.8,A_7=2.0 (Kcal/mol). The catalytic site was estimated to be present between the subsite 5 and 6.