Joint Studies on Dextran-Producing and -Degrading Enzymes

右旋糖酐生产和降解酶的联合研究

基本信息

  • 批准号:
    10044190
  • 负责人:
  • 金额:
    $ 3.2万
  • 依托单位:
  • 依托单位国家:
    日本
  • 项目类别:
    Grant-in-Aid for Scientific Research (B).
  • 财政年份:
    1998
  • 资助国家:
    日本
  • 起止时间:
    1998 至 1999
  • 项目状态:
    已结题

项目摘要

Dextransucrase, one of glucansucrases, catalyzes the formation of dextran from sucrose. Recently Dr. Kim has established a novel mutation technique, Vacuum UV radiation. The mutant strains obtained hyper-produced dextransucrase constitutively, giving a possibility to produce the large amount of dextran. Dextran and its oligosaccharides have been found to have several useful functions to human. In the international project we tried to elucidate the relationship between structures and functions of dextran-producing and -degrading enzymes, and prepared the dextran and isomaltooligosaccharides by both enzymes.(1) We succeeded in isolation of a hyper-produced dextransucrase gene and its expression in Escherichia coli. The mutated position was found in the promoter region. (2) A gene of isomaltotrio-dextranase was cloned and expressed in E. coli. The α-glucosidase gene was found in the upstream region of this gene. Both genes made a cluster structure, which was controlled by one promoter. (3) The large amount of dextran was prepared by hyper-produced dextransucrase. We examined the effective production method of pure isomaltotriose from dextran using isomaltotrio-dextranase. (4) An enzyme giving tetra- and penta-saccharides from dextran was purified and the properties were investigated. (5) Mechanism-based inactivator for dextranase was designed. Kinetic studies on inactivation indicated that compounds synthesized were found to be novel suicide substrate for dextranase.
葡聚糖蔗糖酶是葡聚糖酶的一种,可以催化蔗糖生成葡聚糖。最近,金博士建立了一种新的突变技术——真空紫外线辐射。突变菌株获得高产葡聚糖蔗糖酶组成,提供了生产大量葡聚糖的可能性。右旋糖酐及其低聚糖已被发现对人体具有多种有益功能。在国际项目中,我们试图阐明右旋糖酐生成酶和降解酶的结构和功能之间的关系,并通过这两种酶制备右旋糖酐和低聚异麦芽糖。(1)成功分离了葡聚糖蔗糖酶高产基因并在大肠杆菌中表达。在启动子区域发现了突变位置。(2)克隆了异麦芽糖三葡聚糖酶基因,并在大肠杆菌中表达。α-葡萄糖苷酶基因位于该基因的上游区域。这两个基因形成了一个由一个启动子控制的簇状结构。(3)过量生产葡聚糖蔗糖酶制备了大量葡聚糖。研究了利用异麦芽糖三葡聚糖酶从葡聚糖中提取纯异麦芽糖的有效方法。(4)从右旋糖酐中纯化出一种生成四糖和五糖的酶,并对其性质进行了研究。(5)设计了基于机理的葡聚糖酶灭活剂。失活动力学研究表明,合成的化合物是新的葡聚糖酶自杀底物。

项目成果

期刊论文数量(30)
专著数量(0)
科研奖励数量(0)
会议论文数量(0)
专利数量(0)
T.Saeki: "Localization of α-Glucosidase in Yeast Cells"J.Apple.Glycosci. 45(3). 281-283 (1998)
T. Saeki:“酵母细胞中 α-葡萄糖苷酶的定位”J. Apple 45(3) (1998)。
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    0
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  • 通讯作者:
木村淳夫: "w-Epoxyalkyl α-glucosideによるグルコシダーゼの親和標識"応用糖質科学. 47(2). (2000)
Atsuo Kimura:“w-环氧烷基 α-葡萄糖苷对葡萄糖苷酶的亲和标记”Applied Glycoscience 47(2) (2000)。
  • DOI:
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    0
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永坂 曜介: "Corticium rolfsiiのグルコアミラーゼに関する研究"応用糖質科学. 46(2). 169-178 (1999)
Yosuke Nagasaka:“Corticium rolfsii 的葡糖淀粉酶的研究”,《应用糖科学》46(2) (1999)。
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  • 影响因子:
    0
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K.Nozaki: "Amylases and Branching Enzyme of Developing Kidney Been Seeds on Native Electrophoretic Gel"J.Apple.Glycosci. 45(2). 117-122 (1998)
K.Nozaki:“在天然电泳凝胶上开发肾种子的淀粉​​酶和分支酶”J.Apple.Glycosci。
  • DOI:
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  • 影响因子:
    0
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CHIBA Seiya其他文献

CHIBA Seiya的其他文献

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{{ truncateString('CHIBA Seiya', 18)}}的其他基金

Structures and Substrate Recognitions of Animal, Botanical and Microbial α-Glucosidases and Their Molecular Evolution
动物、植物和微生物 α-葡萄糖苷酶的结构和底物识别及其分子进化
  • 批准号:
    09460041
  • 财政年份:
    1997
  • 资助金额:
    $ 3.2万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
Analyzes for Mechanism of Substate Recognition and Structure of Sugar Chain in Fungus Crystalline alpha-Glucosidase
真菌结晶α-葡萄糖苷酶的物质识别机制和糖链结构分析
  • 批准号:
    06453164
  • 财政年份:
    1994
  • 资助金额:
    $ 3.2万
  • 项目类别:
    Grant-in-Aid for Scientific Research (B)
New Technique for High Yield Production of Glucose by Using of Mutarotase
变旋酶高产生产葡萄糖新技术
  • 批准号:
    05556011
  • 财政年份:
    1993
  • 资助金额:
    $ 3.2万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research (B)
Studies on Mechanism of Substrate Confirmation, Modification of Function, and Utilization for Carbohydrate-Hydrolase and Synthase
碳水化合物水解酶和合酶的底物确认、功能修饰和利用机制研究
  • 批准号:
    05303010
  • 财政年份:
    1993
  • 资助金额:
    $ 3.2万
  • 项目类别:
    Grant-in-Aid for Co-operative Research (A)
Structure and Expression of Mold Crystalline alpha-Glucosidase
霉菌结晶α-葡萄糖苷酶的结构和表达
  • 批准号:
    04453129
  • 财政年份:
    1992
  • 资助金额:
    $ 3.2万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (B)
Kinetic Studies on Allosteric Properties of Honeybee alpha-Glucosidase
蜜蜂α-葡萄糖苷酶变构特性的动力学研究
  • 批准号:
    02660074
  • 财政年份:
    1990
  • 资助金额:
    $ 3.2万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
New Method Using Bio-Reactor for Production of Isomaltose as Food Stuff
利用生物反应器生产食品异麦芽糖的新方法
  • 批准号:
    01860012
  • 财政年份:
    1989
  • 资助金额:
    $ 3.2万
  • 项目类别:
    Grant-in-Aid for Developmental Scientific Research
Mechanism of Occurrence of Carbohydrate Metabolizing Enzymes in Corn Seeds.
玉米种子中碳水化合物代谢酶的发生机制。
  • 批准号:
    62560067
  • 财政年份:
    1987
  • 资助金额:
    $ 3.2万
  • 项目类别:
    Grant-in-Aid for General Scientific Research (C)
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