New Method Using Bio-Reactor for Production of Isomaltose as Food Stuff

利用生物反应器生产食品异麦芽糖的新方法

• 批准号: 01860012
• 负责人: CHIBA Seiya
• 金额: $ 3.33万
• 依托单位: Hokkaido University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Developmental Scientific Research
• 财政年份: 1989
• 资助国家: 日本
• 起止时间: 1989 至 1991
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/en/grant/KAKENHI-PROJECT-01860012/
• 关键词: Isomalto-dextranase; Isomaltose; Bio-reactor; デキストラン

(1) Isomalto-dextranase was purified from Arthrobacter globiformis for mass production of isomaltose.(2) The enzyme showed to release exo-wise isomaltose from dextran, and to release endo-wise isopanose from pullulan.(3) A single active site of the enzyme was kinetically concluded to catalyze the hydrolyses of both alpha-1, 6- and alpha-1, 4-glucosidic linkage of substrate by using isomaltotriose and panose.(4) Conditions of acid treatment were checked toward dextrans for the production of isomaltose. Treatments of dextrans in 0.2N HCl at 95ﾟC for 4 hours were most suitable.(5) When dextrans were treated in 0.2N HCl at 95ﾟC for 4 hours, all of alpha-1, 2-, alpha-1, 3- and alpha-1, 4-glucosidic linkages were cleaved. Hydrolysis degree of the acid-treated dextran by the enzyme indicated more 90%, resulting in isomaltose more than 70% in yeild.

(1)从球形节杆菌中纯化了异麦芽糖葡聚糖酶，用于大规模生产异麦芽糖。(2)该酶能从葡聚糖中释放外向异麦芽糖，从普鲁兰中释放内向异糖。(3)从动力学上推断出该酶的一个活性位点可以催化底物α - 1,6 -和α - 1,4 -糖苷键的水解，同时使用异麦芽糖三糖和葡萄糖。(4)考察了用右旋糖酐生产异麦芽糖的酸处理条件。右旋糖酐在0.2N HCl中95°C处理4 h最合适。(5)右旋糖酐在95°C的0.2盐酸溶液中处理4小时后，α - 1,2 -、α - 1,3 -和α - 1,4 -糖苷键全部断裂。经酸处理后的葡聚糖经酶水解度可达90%以上，异麦芽糖得率可达70%以上。

项目成果

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