Analysis of quartet lipids in methanogenic bacteria

产甲烷菌中四重脂质的分析

• 批准号: 62560092
• 负责人: NISHIHARA Masateru
• 金额: $ 0.26万
• 依托单位: University of Occupational and Environmental Health
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62560092/
• 关键词: Archaebacteria; Methanogen; Ether lipids; Lipid structure; M.thermoautotrophicum

The strucutres of major tetraether types of lipids in the methanogen methanobacterium thermoautotrophicum were elucidated. The systematic names of these lipids were caldarchaeol, gentiobiosyl caldarchaeol, caldarchaetidyl-X and gentiobiosyl caldarchaetidyl-X (X=ethanol-amine, L-serine and myo-inositol). These lipids were classifies in three (ethanolamine, serins and inositol) groups of quartets, each of which is composed of neutral lipids, glycolipids, phospolipid and phosphoglycolipid.The structural features of the quartet provided useful clues for elucidation and understanding of the complexity of lipid pattern of this organism and biosynthetic hypothesis. The quartet concept was extended to a heptad concept by the addition of the results of structural analysis of diether polar lipids.

阐明了产甲烷菌热自养型产甲烷杆菌中主要四醚类脂类的结构。这些脂质的系统名称为caldarchaeol、gentiobiosyl caldarchaeol、caldarchaetidyl-X和gentiobiosyl caldarchaetidyl-X（X=乙醇胺、L-丝氨酸和肌醇）。这些脂类可分为乙醇胺类、丝氨酸类和肌醇类三组四联体，每组四联体分别由中性脂、糖脂、磷脂和磷酸糖脂组成，这些四联体的结构特征为阐明和理解该生物脂类模式的复杂性和生物合成假说提供了有用的线索。通过添加二醚极性脂质的结构分析结果，将四肽概念扩展为七肽概念。

项目成果

Masateru Nishihara: Biochemistry. 28. 95-102 (1989)

西原正辉：生物化学。

Masateru Nishihara: "Extraction and composition of polar lipids from the archaebacterium, Methanobacterium thermoautotrophicum: effective extraction of tetraether lipids by an acidified solvent." Journal of Biochemistry. 101. 977-1005 (1987)

Masateru Nishihara：“从古细菌热自养甲烷杆菌中提取和组成极性脂质：通过酸化溶剂有效提取四醚脂质。”

Masateru Nishihara: Journal of Lipid Research. 29. 384-388 (1988)

Masateru Nishihara：脂质研究杂志。

Masateru Nishihara: "Quantitative conversion of diether or tetraether phospholipids to glycerophosphoesters by dealkylation with boron trichloride: a tool for structural analysis of archaebacterial lipids." Journal of Lipid Research. 29. 384-388 (1988)

Masateru Nishihara：“通过三氯化硼脱烷基化将二醚或四醚磷脂定量转化为甘油磷酸酯：古细菌脂质结构分析的工具。”

Masateru Nishihara: "Heptads of polar ether lipids of an archaebacterium, Methanobacterium thermoautotrophicum: structure and biosynthetic relationship." Biochemistry. 28. 95-102 (1989)

Masateru Nishihara：“古细菌热自养甲烷杆菌的极性醚脂质七联体：结构和生物合成关系。”