Reduced one Carbon Biosynthetic Pathways of the Methanogen Methanosphaera stadtmanae

产甲烷菌 Methanosphaera stadtmanae 的还原碳生物合成途径

• 批准号: 9017471
• 负责人: Terry Miller
• 金额: $ 14万
• 依托单位: Health Research Incorporated/New York State Department of Health
• 依托单位国家: 美国
• 项目类别: Continuing Grant
• 财政年份: 1991
• 资助国家: 美国
• 起止时间: 1991-03-01 至 1996-02-29
• 项目状态: 已结题
• 来源: https://www.nsf.gov/awardsearch/showAward?AWD_ID=9017471&HistoricalAwards=false
• 关键词: Reduced; Carbon; Biosynthetic; Pathways; Methanogen

This project will study a methanogen with unusual physiological attributes--the inability to produce methane from either carbon dioxide or acetate, but to do so from methanol provided that hydrogen is present. Surprising is the fact that a great deal of carbon of cell components is derived from acetate or carbon dioxide and little from methanol; it is this latter aspect of biosynthesis that is the focus of investigations that will use 13-C labelled acetate or carbon dioxide, followed by high-resolution NMR studies, to elucidate key carbon-entrance pathways using intact cells and cell extracts. Key enzymatic reactions and coenzyme requirements of central reactions of the apparently unusual reduced one-carbon biosynthetic pathway(s) will also be studied. The study of a physiologically-unusual methanogenic archaebacterium, with the goal of understanding the means by which acetate and carbon dioxide carbon are built into cellular components is the focus of this project which involves collaboration between microbiologists and chemists. //

该项目将研究一种具有不寻常生理特性的产甲烷菌， 无法从碳中产生甲烷 二氧化物或乙酸盐，但从甲醇中这样做，条件是 存在氢。 令人惊讶的是， 电池组件的碳来源于醋酸盐或二氧化碳 而甲醇几乎没有;这是生物合成的后一方面， 这是调查的重点，将使用13-C标记 乙酸盐或二氧化碳，然后进行高分辨率NMR研究， 使用完整细胞阐明关键的碳进入途径， 细胞提取物 关键酶促反应和辅酶需求 显然不寻常的一碳还原的中心反应 生物合成途径也将进行研究。 一种生理上不寻常的产甲烷菌的研究 古细菌，目的是了解 醋酸盐和二氧化碳的存在 组件是该项目的重点， 微生物学家和化学家之间的合作。 //