Microbial glycosylation of vitamins and its application

1.一种新的维生素C糖苷的分离与鉴定。研究结果表明：1.麦芽糖和L-抗坏血酸与尼日尔曲霉-葡萄糖苷酶共同作用，可高产率（50%）生成一种新的维生素C衍生物6-O-（-葡萄糖基）-L-抗坏血酸。微生物大量生产维生素B_6-糖苷(i)Sporobolomycessingularis生长培养中β-糖基吡哆醇的形成。从在含有乳糖和吡哆醇的培养基上生长的Sporobolomycessingularis的培养液中分离出结晶形式的5 ′-O-（β-半乳糖基）-吡哆醇、4 ′-O-（β-半乳糖基）-吡哆醇和4 ′-O-（β-二半乳糖基）-吡哆醇，产率接近90%。(ii)尼日尔曲霉生长培养物中β-果糖基吡哆醇的形成。在含有蔗糖和吡哆醇的黑曲霉生长培养基中，发现了一种新的吡哆醇衍生物5 '-o-（-果糖基）-吡哆醇.微生物大量生产糖基核苷。Sporobolomyces singularis产生了大量的-半乳糖苷的5-溴尿苷和其他核苷在培养基上生长的乳糖和这些核苷。

1. Isolation and identification of a new glycoside of vitamin C. A new dervative of vitamin C, 6-0-( -glucosyl)-L-ascorbic acid, was found to be formed in a high yield (50%) when maltose and L-ascorbic acid were incubated with Aspergillus niger -gluco-sidase.2. Microbial mass production of vitamin B_6-glycosides. (i) Formation of -glycosylpyridoxines in growing culture of Sporobolomyces singularis. 5'-0( -galactosyl)-pyridoxine, 4'-0-( -galactosyl)-pyridoxine, and 4'-0-( -digalactosyl)-pyridoxine were isolated in crystalline form in nearly 90% yield from the cultured broth of Sporobolomyces singularis grown on medium containing lactose and pyridoxine. (ii) Formation of -fructosylpyridoxine in growing culture of Aspergillus niger. A new derivative of pyridoxine, 5'-o-( -fructosyl)-pyridoxine, was found to be formed in growing culture containing sucrose and pyridoxine by Aspergillus niger.3. Microbial mass production of glycosylnucleosides. Sporobolomyces singularis yielded large amounts of the -galactosides of 5-bromouridine and other nucleosides in growing cultures on media with lactose and these nucleosides.