Microbial glycosylation of vitamins and its application

1. 麦芽糖和l -抗坏血酸与黑曲霉-葡萄糖苷酶孵育后，可高产出6-0-（-葡萄糖基）- l -抗坏血酸这一新的维生素C衍生物。微生物量产维生素b_6 -糖苷。(i)奇异孢子菌生长培养中-糖基吡哆醇的形成。从含有乳糖和吡哆醇的培养基中分离出5′-0（-半乳糖酰基）-吡哆醇、4′-0-（-半乳糖酰基）-吡哆醇和4′-0-（-双半乳糖酰基）-吡哆醇，提取率接近90%。（ii）黑曲霉生长培养中-果糖嘧啶吡哆醇的形成。在含有蔗糖和吡哆醇的生长培养基中，发现了一种新的吡哆醇衍生物，5′-o-（-果糖基）-吡哆醇。微生物大量生产糖基核苷。在含有乳糖和这些核苷的培养基上，奇异孢子菌产生了大量的5-溴吡啶半乳糖苷和其他核苷。

1. Isolation and identification of a new glycoside of vitamin C. A new dervative of vitamin C, 6-0-( -glucosyl)-L-ascorbic acid, was found to be formed in a high yield (50%) when maltose and L-ascorbic acid were incubated with Aspergillus niger -gluco-sidase.2. Microbial mass production of vitamin B_6-glycosides. (i) Formation of -glycosylpyridoxines in growing culture of Sporobolomyces singularis. 5'-0( -galactosyl)-pyridoxine, 4'-0-( -galactosyl)-pyridoxine, and 4'-0-( -digalactosyl)-pyridoxine were isolated in crystalline form in nearly 90% yield from the cultured broth of Sporobolomyces singularis grown on medium containing lactose and pyridoxine. (ii) Formation of -fructosylpyridoxine in growing culture of Aspergillus niger. A new derivative of pyridoxine, 5'-o-( -fructosyl)-pyridoxine, was found to be formed in growing culture containing sucrose and pyridoxine by Aspergillus niger.3. Microbial mass production of glycosylnucleosides. Sporobolomyces singularis yielded large amounts of the -galactosides of 5-bromouridine and other nucleosides in growing cultures on media with lactose and these nucleosides.