Enzymatic Glycosylation and Phosphatidylation in Organic Solvent Systems.

1.脂溶性生理活性化合物在亲水性有机溶剂中的酶促糖基化：当含有这些萜醇和纤维二糖（或乳糖）的50%乙腈混合物与杏仁β-葡糖苷酶（或曲霉β-半乳糖苷酶）一起孵育时，发现形成了长无环萜醇的新衍生物，O-β-葡糖基法呢醇、O-β-半乳糖基法呢醇和O-β-半乳糖基香叶基香叶醇。酚类化合物的糖基化--一种对芳香族化合物羟基的高转糖基化反应，发现嗜热脂肪芽孢杆菌环麦芽糖糊精葡聚糖基转移酶通过转糖基化反应合成苯基-α-葡萄糖苷和O-α-葡萄糖基儿茶酚，并以晶体形式分离和鉴定了这些葡萄糖苷。在过氧化物酶-过氧化氢-氧化体系中，这些糖苷比苷元稳定得多; 3.有机溶剂中水溶性维生素的酶促磷脂化研究发现链霉菌磷脂酶D催化1，2-二棕榈酰-3-sn-磷脂酰胆碱（DPPC）的二棕榈酰磷脂酰残基转移到水溶性维生素（硫胺素、泛酸和这些相关化合物）在两相系统中进行，以分别提供维生素的新型脂溶性衍生物（DPP-硫胺素、DPP-泛酸、DPP-硫胺素-丙基二硫化物、DPP-呋硫硫胺素和DPP-泛酸基乙基醚）。这些新的衍生物进行了分离和鉴定。

1.Enzymatic glycosylation of fat-solubly physiologically active compounds in a hydrophilic organic solvent.New derivatives of long acyclic terpene alcohols, O-beta-glucosylfarnesol, O-beta-galactosylfarnesol and O-beta-galactosylgeranylgeraniol, were found to be formed, when the mixture containing these terpene alcohols and cellobiose (or lactose) in 50 % acetonitrile was incubated with almond beta-glucosidase (or Aspergillus oryzae beta-galactosidase). These glycosylated derivatives were isolated and identified.Glucosylation of phenolic compounds-A high transglucosylation reaction to hydroxy residue of aromatic compounds.The synthesis of pheny1-alpha-glucoside and 0-alpha-glucosylcatechol via transglucosylation reaction by Bacillus stearothermophilus cyclomaltodextrin glucanotransferase was found. These glucosides were isolated in crystalline form and identified. These glucosides were much more stable than the aglycones in a peroxidase-hydrogen peroxide-oxidizing system.3.Enzymatic phosphatidylation of water-soluble vitamins in organic solvents.Streptomyces sp. phospholipase D was found to be catalyze the transfer reaction of the dipalmitoylphosphatidyl residue from 1,2-dipalmitoy1-3-sn-phosphatidy1-choline (DPPC) to water-soluble vitamins (thiamin, pantothenic acid, and these related compounds) in a biphasic system, to afford novel fat-soluble derivatives of the vitamins (DPP-thiamin, DPP-pantothenic acid, DPP-thiamin-propyldisulfide, DPP-fursulthiamin, and DPP-pantothenyl ethyl ether), respectively. These novel derivatives were isolated and identified.