STUDIES ON TOXIC AGENT IN LOWER BENTHIC ANIMALS INHABITING IN THE SETO INLAND SEA, ESPECIALLY ON TETRODOTOXIN OF THE FLATWORM

濑户内海低等底栖动物有毒物质特别是扁虫河豚毒素的研究

• 批准号: 62560211
• 负责人: MIYAZAWA Keisuke
• 金额: $ 1.34万
• 依托单位: HIROSHIMA UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62560211/
• 关键词: Tetrodotoxin; distribution of toxicity; Echinodermata; Platyhelminthes; 棘皮動物; 紐形動物; テトロドトキシン; 部位別毒生

In order to clarify the distribution, origin and mechanism of accumulation of tetrodotoxin (TTX) in the lower benthic animals in the Seto Inland Sea, some examinations were performed and following results were obtained.1. Among the various organs of the starfish Astropecten polyacanthus, ovary showed the highest potency (max. 12700 MU/g) followed by digestive organs. As the result, overall toxicity was remarkably higher in female than in male.2. Among the various tissues of the flatworm planocera multitentaculate, oviduct was most toxic, followed by digestive organs. The egg masses laid exhibited extremely high potency upto 10700 MU/g. These rusults suggested that the flatworm and the starfish possess TTX as defense or alarm substance against predators. Some TTX-producing bacteria were isolated from the digestive tract of flatworm.3. Ribbon worms, Lineus fuscoviridis, Tubulanus punctatus and some others, had lethal potencise. The toxios were partially purified and identified as TTX and its relating substances by TLC, HPLC and GC-MS. This may be the first finding of TTX in Nemertini.

为了明确河豚毒素(TTX)在濑户内海低层底栖动物中的分布、来源和积累机制，进行了一些研究，得到了以下结果： 1．在多棘海星的各个器官中，卵巢的效力最高（最大 12700 MU/g），其次是消化器官。结果表明，女性总体毒性明显高于男性。2．多触扁虫的各个组织中，输卵管毒性最强，其次是消化器官。产下的卵块表现出极高的效力，高达 10700 MU/g。这些结果表明，扁虫和海星拥有 TTX 作为针对捕食者的防御或警报物质。从扁形虫消化道中分离得到一些产TTX细菌。 3．带状蠕虫、褐褐色线虫、点状管虫和其他一些蠕虫具有致死效力。毒物经部分纯化，经TLC、HPLC、GC-MS鉴定为TTX及其有关物质。这可能是 Nemertini 中首次发现 TTX。

项目成果

K. Miyazawa;M. Higashiyama;K. Ito;T. Noguchi;O. Arakawa;Y. Shida;K. Hashimoto: Toxicon.

K.宫泽；M.

K.Miyazawa;J.K.Jeon;T.Noguchi;K.Ito;K.Hashimoto: TOXICON. 25. 975-980 (1987)

K.宫泽；J.K.Jeon；T.Noguchi；K.Ito；K.Hashimoto：TOXICON。

T.Noguchi,etal.K.Miyazawa.: Progress in venom and toxin research. 336-347 (1987)

T.Noguchi,etal.K.Miyazawa.：毒液和毒素研究进展。

J.K. Jeon;K. Miyazawa;T. Noguchi;H. Narita;s. Matsubara;M. Nara;K. Ito;K. Hashimoto: Nippon Suisan Gakkaishi. 52. 1065-1069 (1986)

J.K.

K.Miyazawa;M.Higashiyama;K.Hori;T.Noguchi;K.Ito;K.Hashimoto: MARINE BIOLOGY. 96. 385-390 (1987)

K.宫泽；M.东山；K.堀；T.野口；K.伊藤；K.桥本：海洋生物学。