Studies on the production of prostaglandin E2(PGE2) and its related substances in red algae of the genus Gracilaria

江蓠属红藻产前列腺素E2(PGE2)及其相关物质的研究

• 批准号: 10660196
• 负责人: MIYAZAWA Keisuke
• 金额: $ 2.56万
• 依托单位: Hiroshima University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for Scientific Research (C)
• 财政年份: 1998
• 资助国家: 日本
• 起止时间: 1998 至 1999
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10660196/
• 关键词: Gracilaria verrucosa; marine algae poisoning; prostaglandin; arachidonic acid; prostaglandin E2; red algae; 紅藻オゴノリ; オゴノリ科紅藻; 食中毒; PGE2

There have been three official poisoning incidents in Japan, including three deaths, due to the ingestion of raw red algae of the genus Gracilaria have occurred in Japan. In October 1993, a food poisoning incident due to the ingestion of 'ogonori'(Gracilaria verrucosa), an edible red alga occurred at Yokohama, Kanagawa Prefecture. Japan, resulting in two victims, including one death (female). No causative agent present in the ogonori was found from a routine bioassay for marine toxins. Responsibility of prostaglandins(PGs), particularly mainly PGE2, for the "ogonori" poisoning has been suggested. This type of poisoning is very unusual, and differs from the more familiar forms of poisoning occurring after ingestion of marine organisms.From a food hygienic point of view, the production of PGE2 and its related substances was examined in five species of red algae such as G.verrucosa, G.bursa-pastoris, Gracilariopsis chorda, G. textorii and Gracilaria sp. in Seto Inland Sea, Japan in this research project. In the specimens of G.verrucosa, 0.1-57.9μg/g of PGE2 was found in fresh alga and, on addition of araohidonate, its amount increased from 1.8 to 90μg/g. The production of increased amount of PGE2 from the specimens treated with liquid nitrogen was several times larger in comparison with that of untreated speoimens. But the production of PGE2 was extremely decreased by acid treatment of the algae. In the red algae except for G.verrucosa, PGE2 was not detected. However, unidentified PGE2 related substances were detected, whose UV absorption maxima was near 240nm. One of these gave rise to a positive ESI mass spectrum, showing a protonated molecule ion of (M+H)ィイD1+ィエD1 at m/z 372 and other ions of (M+H-HィイD22ィエD2O)ィイD1+ィエD1 at m/z 355, (M+H-2HィイD22ィエD2O)ィイD1+ィエD1 at m/z 337, (M+H-3HィイD22ィエD2O)ィイD1+ィエD1 at m/z 319 and (M+H-4HィイD22ィエD2O)ィイD1+ィエD1 at m/z 301.

日本已经发生了三起官方中毒事件，其中包括三人死亡，原因是日本发生了江蓠属的生红藻。1993年10月，在神奈川县横滨市发生了一起因食用红藻“小尾”（江蓠）而引起的食物中毒事件。日本，造成两名受害者，其中一人死亡（女性）。海洋毒素的常规生物测定中没有发现小尾虫中存在的病原体。已经提出了前列腺素（PGs），特别是主要是PGE 2，对“ogonori”中毒的责任。从食品卫生学的角度出发，研究了多疣海藻、囊状海藻、江蓠、江蓠、绿藻、textorii和龙须菜属Gracilaria sp.。在疣状海鞘中，新鲜海鞘中含有0.1-57.9μg/g的PGE_2，加入阿糖胞苷后，其含量由1.8 μ G/g增加到90μg/g。经液氮处理的标本产生的PGE_2的量比未经处理的标本增加数倍。而酸处理则显著降低了PGE_2的产生。在红藻中，除疣状革兰氏阴性菌外，未检测到PGE 2。然而，检测到未鉴别的PGE 2有关物质，其UV吸收最大值接近240 nm。其中之一产生正ESI质谱，显示在m/z 372处的质子化分子离子（M+H）H2 D1+ H2 D2 D1和在m/z 372处的其他离子（M + H）H2 D2 D1+ H2 D2 D1）。（M+H-H-吡喃D22吡喃D2 O）吡喃D1+吡喃D1，m/z 355，（M+H-2 H-吡喃D22-吡喃D2 O）吡喃D1+吡喃D1，m/z 337，（M+H-3 H-吡喃D22-吡喃D2 O）吡喃D1+吡喃D1，m/z 319和（M+H-4 H-吡喃D22-吡喃D2 O）吡喃D1+吡喃D1，m/z 301。