Structure and Function of Telencephalon-Specific Antigen

在兔脑的免疫组织化学分析中，单克隆抗体（MAb） 271A6选择性地标记了所有端脑区域的灰质，而抗体不标记非端脑区域。利用MAb 271A6亲和柱，从兔脑中分离出抗原271A6（端脑特异性抗原）。端脑特异性抗原是一种分子量为500 KDa的膜蛋白，由4个130 KDa的亚基组成。端脑特异性抗原的每个亚基含有7或8个n-天冬酰胺连接的低聚糖链。对纯化的端脑特异性抗原产生抗血清。抗血清对兔脑的免疫组化染色模式与单抗271A6几乎相同。这表明端脑特异性抗原的整个核心蛋白在端脑区选择性表达。利用抗血清对不同哺乳动物的大脑进行免疫组织化学检测。该抗血清在小鼠、大鼠、豚鼠、猫、猴子和家兔的端脑区灰质中均有标记，提示该端脑特异性抗原在哺乳动物中具有保守性。基于上述结果，我们将端脑特异性抗原命名为“端脑蛋白”。我们现在正在确定端脑蛋白的初级结构和检查端脑蛋白的功能作用。

In immunohistochemical analysis of the rabbit brain, monoclonal antibody (MAb) 271A6 selectively labels the gray matter of all telencephalic regions whereas the antibody leaves non-telencephalic regions unlabeled. Using affinity columns with MAb 271A6, we isolated the antigen 271A6 (telencephalon-specific antigen) to homogeneity from rabbit brain. The telencephalon-specific antigen turned out to be a membrane protein with a molecular weight of 500 KDa, consisting of four 130 KDa subunits. Each subunit of the telencephalon-specific antigen contained 7 or 8 N-asparagine-linked oligosaccharide chains.An antiserum was generated against the purified telencephalon-specific antigen. The pattern of the immunohistochemical staining of the rabbit brain with the antiserum was almost identical to that with MAb 271A6. This indicates that entire core protein of the telencephalon-specific antigen is selectively expressed in telencephalic regions. Using the antiserum, we examined immuno-histochemically the brains of various mammalian species. The antiserum labeled the gray matter of telencephalic regions of mice, rats, guinea pigs, cats, and monkeys in addition to rabbits, suggesting that the telencephalon-specific antigen is conserved among mammalian species.Based on above results, we termed the telencephalon-specific antigen "Telencephalin". We are now determining the primary structure of Telencephalin and examining the functional roles of Telencephalin.