Molecular mechanisms invovlved in sexual differentiation of the rat brain

大鼠脑性别分化的分子机制

• 批准号: 62570071
• 负责人: SAKUMA Yasuo
• 金额: $ 1.09万
• 依托单位: HIROSAKI UNIVERSITY (1988)Niigata University (1987)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570071/
• 关键词: Lordosis; Midbrain central gray; Nerve growth factor; Sexual dimorphism; Testosterone; 中脳中心灰白質; テストステロン; ロード-シス; 逆行性興奮; 活動電位; 腹内側核; 性行動; エストロジェン; 脳の雌雄差; 性ホルモン; 生殖行動

Injection of antibody to nerve growth factor into the cerebral lateral ventricle blocked testosterone-induced behavioral defeminization of neonatal female rat. When tested as adults following ovariectomy and the combined estrogen-progesterone treatment, the injected animals showed a significantly higher lordosis quotient than the testosterone-treated, normal rebbit serum infused controls. Failure of vaginal opening and clitoral enlargement manifested the well-documented masculinizing effect of testosterone on the genitalia in the experimental as well as the control animals. Estrogen-sensitivity of hypothalamic neurons which are responsible for the induction of lordosis was retained in the experimental animals. Recordings of the antidromic action potentials from neurons in the ventromedial nucleus of the hypothalamus following stimulation of the midbrain central gray revealed that estrogen decreased the antidromic activation threshold and shortened the absolute refractory period of th e hypothalamic efferents along with the estrogen-induced behavioral activation in the experimental animals. In the control group, the estrogen-induced neuronal activation was lost altogether with the behavioral activation.

侧脑室注射神经生长因子抗体阻断睾酮诱导的雌性新生大鼠行为性去女性化。在卵巢切除术和雌激素-孕酮联合治疗后作为成年动物进行测试时，注射的动物显示出比睾酮治疗的正常rebbit血清输注对照组显著更高的脊柱前凸商。在实验动物和对照动物中，阴道张开和阴蒂增大的失败证明了睾丸激素对生殖器的雄性化作用。雌激素敏感性的下丘脑神经元，负责诱导脊柱前凸保留在实验动物。刺激中脑中央灰质后，记录下丘脑腹内侧核神经元的逆向动作电位，发现雌激素可使实验动物下丘脑传出神经元的逆向激活阈值降低，绝对不应期沿着缩短。在对照组中，雌激素诱导的神经元激活与行为激活一起丧失。

项目成果

Yanase, Masahiro: "Nerve growth factor mediated sexual differentiation on the rat hypothalamus." Neuroscience Research. 6. 181-185 (1988)

Yanase, Masahiro：“神经生长因子介导大鼠下丘脑的性别分化。”

Akaishi, Takao: "Effect of estrogen on hypothalamic paraventricular nucleus neurons in tissue slices from ovariectomized rats." Biomedical Research. 10. (1989)

Akaishi，Takao：“雌激素对卵巢切除大鼠组织切片中下丘脑室旁核神经元的影响。”

Sakuma, Y.: Journal of Neurophysiology. 57. 1148-1159 (1987)

佐久间 Y.：神经生理学杂志。

Akaishi,Takao.: Biomedical Research. 10. (1989)

赤石高雄：生物医学研究。

Takahashi, Yasuo: "Brain aromatase activity and behavioral consequences in the male rat treated in utero with 4 hydroxy-androstenedione." Endocrinologia Japonica. 36. (1989)

Takahashi, Yasuo：“在子宫内接受 4 羟基雄烯二酮治疗的雄性大鼠的脑芳香酶活性和行为后果。”