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The Sexual Differentiation of the Rat Hypothalamus: Organizational and Activational Effects of Sex Steroids

大鼠下丘脑的性别分化：性类固醇的组织和激活作用

基本信息

批准号：
60570077
负责人：
SAKUMA Yasuo
金额：
$ 1.02万
依托单位：
Niigata University
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1985
资助国家：
日本
起止时间：
1985 至 1986
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-60570077/
关键词：
Antidromic action potential Estrogen Hypothalamus Lordosis Midbrain oxytocin Paraventricular nucleus ロードシス.

项目摘要

The ventromedial nucleus of the hypothalamus (VMN) is associated with estrogen-dependent neural regulation of autonomic, neuroendocrine and behavioral functions. VMN cells with axons to the midbrain central gray (CG) have been demonstrated as one of the neural substrates participating in these regulatory processes. The aim of the present series of experiments was to identify and characterize estrogen-sensitive VMN neurons with axons projecting to the CG. Antidromic action potentials were evoked in VMN cells following CG stimulation in the female and male rats, which received various endocrine treatments. Estrogen treatment of adults significantly decreased the antidromic activation threshold and shortened the refractory period in female rats or neonatally castrated male rats, but not in males or females exposed neonatally to testosterone. In the female rats which received no neonatal treatment, estrogen-sensitive neurons were localized in the rostral VMN, had large soma with axonal pro … More jections to the CG via the zona incerta or the supraoptic commissure.A similar type of sexual dimorphism in the estrogen sensitivity existed in the hypothalamic paraventricular nucleus (PVN). Extracellular antidromic potentials were recorded from the PVN of the male and female rat following electrical stimulation of the neurohypophysis. Effects of estrogen-treatment in the castrated animals were investigated after classifying the antidromically identified cells into tonically or phasically-firing, or silent groups according to their patterns of spontaneous discharge. Estrogen significantly decreased the antidromic activation threshold and shortened the refractory period as well as the antidromic spike latency in the tonically-firing cells in the female but not those in the male.Hypothalamic tissue slices containing the PVN were obtained from the ovariectomized female rat. The slices were perfused by aerated artificial cerebrospinal fluid. Acute effects of estrogen were examined on the firing rate as well as on the modulatory responses induced by biogenic amines. Among 51 cells tested, addition of estrogen to the perfusate caused immediate activation in 7 and inhibition in 23 cells, respectively. In a group of estrogen-insensitive cells, simultaneous administration of estrogen with noradrenaline or dopamine resulted in augmented responses to these substances. These data indicated potential estrogen action at the neuronal membrane, to control the activity of specific groups of the hypothalamic cells. Less

下丘脑腹内侧核（VMN）与雌激素依赖的自主神经、神经内分泌和行为功能的神经调节有关。VMN细胞与中脑中央灰质（CG）的轴突已被证明是参与这些调节过程的神经基质之一。本系列实验的目的是识别和表征雌激素敏感的VMN神经元的轴突投射到CG。在接受各种内分泌治疗的雌性和雄性大鼠中，CG刺激后在VMN细胞中诱发逆向动作电位。雌激素治疗的成年显着降低了逆向激活阈值，缩短了不应期雌性大鼠或卵巢去势雄性大鼠，但不暴露于睾丸激素的男性或女性。在未接受新生治疗的雌性大鼠中，雌激素敏感神经元位于VMN的嘴侧，具有大的索马体，轴突突起， ...更多信息雌激素敏感性的性别二型性在下丘脑室旁核（PVN）也存在。电刺激神经垂体后，从雄性和雌性大鼠的PVN记录细胞外逆向电位。雌激素治疗的去势动物的影响进行了研究后，逆向识别的细胞分为强直性或相位放电，或沉默组根据其模式的自发放电。雌激素可显著降低雌性大鼠的张力放电细胞的逆向激活阈值，缩短不应期和逆向放电潜伏期，但对雄性大鼠无显著影响。采用充气人工脑脊液灌注脑片。雌激素的急性效应进行了研究，以及对生物胺诱导的调制反应的放电率。在51个细胞中，加入雌激素的灌流液引起立即激活7个细胞和抑制23个细胞，分别。在一组雌激素不敏感的细胞中，同时给予雌激素和去甲肾上腺素或多巴胺导致对这些物质的反应增强。这些数据表明，潜在的雌激素作用在神经元膜，控制下丘脑细胞的特定群体的活动。少