Analysis of serotonergic system in the respiratory and circulatory regulation of the medullary raphe nuclei.

中缝髓核呼吸和循环调节中血清素能系统的分析。

• 批准号: 62570091
• 负责人: SUGA Toshio
• 金额: $ 1.34万
• 依托单位: Saitama Medical University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570091/
• 关键词: Medullary raphe nuclei; Respiratory regulation; Circulatory regulation; REspiratory neuron; Rostral ventrolateral medulla; Serotonergic system; GABAergic system; Microiontophoretic application; 吻側腹外側延髄; 5-HT_1受容体; 5-HT_2受容体; 5ーHT_1受容体; 5ーHT_2受

Respiratory and circulatory regulation mechanisms of the medullary raphe nuclei, that is, nucleus raphe pallidus,obscurus and magnus, were investigated, especially concerning their serotonergic and GABA ergic mechanisms, using microiontophoretic application methods.1. Electrical stimulation of medullary raphe nuclei of the cat produced almost parallel depressions of respiration (phrenic nerve), renal nerve and blood pressure, but, when the stimulating electrode advanced in the more ventral part of the nuclei, these responses moved gradually to the excitatory ones.These responses were analyzed on the averaged potential of the phrenic nerve elicited by raphe stimulation. This averaged potential was composed of the initial slight excitatory potential and following long-lusting depressant one, the former becoming larger as the stimulating site was more ventral, and producing excitatory response by repetitive stimulation. The former potential was depressed by methysergide (5-HT_2 antagonist … More ) and the latter was reduced by propranolol (recognized as possessing 5-HT_1 antagonistic effect).2. Medullary respiratory neurons (inspiratory and expiratory neurons) and vasomotor neurons in the rostral ventrolateral medulla were depressed by raphe stimulation with ghort latency, and these depressant responses were antagonized by intravenous or microiontophoretic application of picrotoxin, bicuculin (GABA antagonists) and propranolol, suggesting projection of serotonergic and GABAnergic systems of raphe nuclei to these neurons.3. These respiratory and vasomotor neurons were depressed dose-dependently by iontophoretic application of serotonin and GABA, and these depressant effects were antagonized by simultaneous application of 5-HT_1 - and GABA antagonists. But, application of methysergide rather augmented the depressant effect of serotonin, suggesting co-localization of 5-HT_2 receptor in addition to 5-HT_1 receptor.4. These effects produced by raphe stimulation and serotonergic and GABAergic drugs were observed similarly in the medullary non-respiratory and non-vasomotorneurons, too, suggesting pan-depressant control of raphe nuclei to the medullary neurons. Less

用微离子电泳法研究了延髓中缝核，即中缝苍白核、隐核和大中缝核的呼吸和循环调节机制，特别是5-羟色胺能和GABA能机制。电刺激猫延髓中缝核团引起呼吸(膈神经)、肾神经和血压几乎平行的抑制，但当刺激电极向核团腹侧推进时，这些反应逐渐移向兴奋性反应。这一平均电位由最初的轻微兴奋电位和随后的长欲抑制电位组成，前者随着刺激部位越向腹侧而变大，并通过重复刺激产生兴奋反应。5-羟色胺(5-HT2)拮抗剂…可抑制前一种电位后者可被心得安(公认具有5-HT1拮抗作用)减少。延髓头端腹外侧区的呼吸神经元(吸气神经元和呼气神经元)和血管运动神经元均受到中缝刺激的抑制，这些抑制反应可被静脉或微离子导入应用印防己毒素、荷包牡丹碱(GABA拮抗剂)和心得安拮抗，提示中缝核团的5-羟色胺能和GABA能系统向这些神经元投射。5-羟色胺和GABA对这些呼吸和血管运动神经元的抑制作用呈剂量依赖性，同时应用5-HT1和GABA拮抗剂可拮抗这种抑制作用。但应用甲基丝裂原可增强5-羟色胺的降压作用，提示5-HT2受体与5-HT1受体共存。在延髓的非呼吸性和非血管运动神经元中，同样观察到刺激中缝核团和5-羟色胺能和GABA能药物所产生的这些效应，提示中缝核团对延髓神经元具有泛抑制作用。较少

项目成果

Kuniyoshi Kumaido: "Research on the serotonergic mechanisms in the respiratory regulation through the mediation of medullary raphe nuclei in the cat" Japanese Journal of Pharmacology. 43(Suppl). 80 (1987)

Kuniyoshi Kumaido：“通过猫中缝髓核介导的呼吸调节中的血清素机制的研究”《日本药理学杂志》。

小倉弘章,田中求,須賀俊郎: 埼玉医科大学雑誌. 15(1). 46-47 (1988)

Hiroaki Ogura、Motomu Tanaka、Toshiro Suga：埼玉医科大学学报 15(1) (1988)。

Kuniyoshi Kumaido;Motomu Tanaka;Masahiko Suzuki and Toshio Suga: Japanese Journal of Pharmacology. 43. 80 (1987)

Kuniyoshi Kumaido；Motomu Tanaka；Masahiko Suzuki 和 Toshio Suga：日本药理学杂志。

Kuniyoshi Kumaido: "Research on the serotonergic mechanisms in the respiratory regulation through the mediation of medullary raphe nuclei in the cat" Japanese Journal of Pharmacology. 43(Suppl). 80P (1987)

Kuniyoshi Kumaido：“通过猫中缝髓核介导的呼吸调节中的血清素机制的研究”《日本药理学杂志》。

"Participation of serotonergic and GABAergic system in the circulatory regulation of the medullary raphe nuclei" Progress in Nerve Research 34 (5) 1990.

“血清素能和 GABA 能系统参与中缝髓核循环调节”神经研究进展 34 (5) 1990。