Dynamic respiratory regulation in plant mitochondria

植物线粒体的动态呼吸调节

• 批准号: 237658134
• 负责人: Professor Dr. Markus Schwarzländer
• 金额: --
• 依托单位: Arbeitsgruppe Plant Energy Biology
• 依托单位国家: 德国
• 项目类别: Independent Junior Research Groups
• 财政年份: 2013
• 资助国家: 德国
• 起止时间: 2012-12-31 至 2019-12-31
• 项目状态: 已结题
• 来源: https://gepris.dfg.de/gepris/projekt/237658134?language=en
• 关键词: Dynamic; respiratory; regulation; plant; mitochondria

In plants mitochondrial respiration is critical for cellular ATP provision, synthesis of metabolites and efficient photosynthesis. The ability to flexibly adjust respiratory metabolism is particularly important in plants to maintain growth and development in a changeable environment. The way in which such dynamic regulation of mitochondrial respiration is achieved is poorly understood. In the proposed research the regulation of respiration in response to changes in mitochondrial thiol redox status and calcium levels will be investigated. Novel genetically-encoded fluorescent sensors will be exploited to assess mitochondrial function with high resolution by quantitative live imaging techniques. In vivo microscopy of the model plant Arabidopsis will be combined with biochemical studies in vitro and in organello to monitor changes in mitochondrial redox, calcium and energy status, to identify regulators that link thiol redox regulation and calcium dynamics with respiration and to dissect control mechanisms using mutant lines. From the available data a model of respiratory dynamics in single mitochondria will be generated, to predict as yet unknown regulatory properties. The results will provide novel insights into organellar bioenergetic control in vivo and open the door to an in depth understanding of mitochondrial regulation in plants.

在植物中，线粒体呼吸对于细胞ATP供应、代谢产物的合成和有效的光合作用至关重要。灵活调节呼吸代谢的能力对于植物在多变的环境中维持生长发育尤为重要。线粒体呼吸的这种动态调节是如何实现的，目前还知之甚少。在拟议的研究中，将研究呼吸对线粒体巯基氧化还原状态和钙水平变化的调节。新的基因编码的荧光传感器将被利用来评估线粒体功能与定量活成像技术的高分辨率。在体内显微镜的模式植物拟南芥将结合在体外和organello的生化研究，以监测线粒体氧化还原，钙和能量状态的变化，以确定调节硫醇氧化还原调节和钙动力学与呼吸和解剖控制机制，使用突变株系。从现有的数据中，将生成单个线粒体中的呼吸动力学模型，以预测尚未未知的调节特性。这些结果将为体内细胞器生物能量控制提供新的见解，并为深入了解植物线粒体调控打开大门。

项目成果

The EF-Hand Ca2+ Binding Protein MICU Choreographs Mitochondrial Ca2+ Dynamics in Arabidopsis[OPEN]

• DOI: 10.1105/tpc.15.00509
• 发表时间: 2015-11
• 期刊: Plant Cell
• 影响因子: 11.6
• 作者: Stephan Wagner;Smrutisanjita Behera;Sara De Bortoli;D. Logan;Philippe Fuchs;Luca Carraretto;E. Teardo;L. Cendron;Thomas Nietzel;Magdalena Füßl;F. G. Doccula;L. Navazio;M. Fricker;Olivier Van Aken;I. Finkemeier;A. Meyer;I. Szabó;Alex Costa;M. Schwarzländer

Redox-mediated kick-start of mitochondrial energy metabolism drives resource-efficient seed germination

• DOI: 10.1073/pnas.1910501117
• 发表时间: 2020-01-07
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Nietzel, Thomas;Mostertz, Joerg;Schwarzlaender, Markus
• 通讯作者: Schwarzlaender, Markus

The mitochondrial monothiol glutaredoxin S15 is essential for iron-sulfur protein maturation in Arabidopsis thaliana

• DOI: 10.1073/pnas.1510835112
• 发表时间: 2015-11-03
• 期刊: PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
• 影响因子: 11.1
• 作者: Moseler, Anna;Aller, Isabel;Meyer, Andreas J.
• 通讯作者: Meyer, Andreas J.

Multiparametric real-time sensing of cytosolic physiology links hypoxia responses to mitochondrial electron transport.

• DOI: 10.1111/nph.16093
• 发表时间: 2019-09
• 期刊: The New phytologist
• 作者: Stephan Wagner;Janina Steinbeck;Philippe Fuchs;Sophie Lichtenauer;Marlene Elsässer;Jos H. M. Schippers;Thomas Nietzel;Cristina Ruberti;Olivier Van Aken;A. Meyer;Joost T. van Dongen;Romy R. Schmidt;M. Schwarzländer