Pharmacological study on intracerebral GABA mechanism

1.GABA免疫组织化学。(1)脊髓内GABA神经元的研究大鼠脊髓内的GABA神经元发出黄绿色FITC特异性荧光。在脊髓前角和后角观察到CABA阳性细胞和GABA阳性纤维的特殊荧光定位。为了比较GABA在脊髓内的分布和合成酶的分布，我们用GAD抗体用间接荧光抗体技术制备了标本，并在同一水平上研究了其分布状态。结果发现，GAD的分布与GABA的定位一致，但荧光强度和分布密度小于GABA的特异性荧光。因此，我们认为直接使用GABA抗体在组织化学上更准确；寻找GABA阳性细胞和阳性纤维。(2)脑内GABA神经元的研究。我们用免疫组织化学方法研究了…中黑质-纹状体的GABA通路。将其与GAD定位进行比较，并与GAD定位进行比较。结果，GABA阳性细胞和阳性纤维在该层内发出绿色荧光。对同一水平的GAD阳性细胞和阳性纤维的研究表明，它们的分布与GABA的分布基本一致。GABA阳性纤维在黑质致密区弥漫发出绿色荧光。GAD阳性细胞在同一水平上的分布符合GABA分布。脑组织中GABA含量的生化分析。将加入反应试剂的大鼠脑组织样品经前处理后注入10-25ul的高效液相色谱柱中。如果进样量超过25ul，峰宽可能变得太大，在254 nm 0.01AUF处约为20-25 ng。脑组织样品的标准曲线为18 ng/25ul，保留时间为3.40分钟。总量为400微升，即288毫微克/400微升。因此，样品中GABA的含量为2.88微克/毫升。在用高效液相色谱法分析脑内GABA时，在本实验中，一个可靠和可能的值是2微克/毫升左右，但对它的研究仍在进行中。较少

1. GABA immunohistochemistry. (1) Study of intraspinal GABA neuron The GABA neuron in the rat spinalcord issued yellowishgreen FITC-specific fluorescence. Specific fluorescence showing the localization of CABA-positive hells and GABA-positive fibers was observed in the spinal anterior horn and posterior horn. To compare the distribution of intraspinal GABA with that of a synthetic enzyme, we prepared specimens by an indirect fluorescent antibody technique using a GAD antibody, and studied for the distribution state on the same level. As a result, we found that the GAD distribution agreed with GABA localization, but the fluorescent intensity and distribution density were less than those of the specific fluorescence of GABA. Accordingly, we believe that using a GABA antibody directly is more accurate in histochemical ; searches for GABA-positive cells and positive fibers. (2) Study of intracerebral GABA neuron. We have studied immunohistochemically the nigra-striatal GABA pathway in the … More rat, and compared it with GAD localization, and compared it with GAD localization. As a result, GABA-positive cells and positive fibers issued green fluorescence in the stratum. Study of GAD-positive cells and positive fibers on the same level revealed that their distribution almost agreed with GABA distribution. GABA- positive fibers issued green fluorescence diffusedly at substantia nigra zoma compacta. The distribution of GAD-positive cells on the same level agreed with GABA distribution.2. Biochemical analysis of GABA content in brain tissues. After pretreatment, the rat brain tissue sample to which a reaction reagent had been added was injected into 10-25ul HPLC. The peak width may become too large if the injection amount exceeds 25ul, with about 20-25 ng at 254 nm 0.01 AUF. The brain tissue sample showed a value of 18 ng/25 ul by a standard curve, and the retention time was 3.40 minutes. The total amount was 400 ul, which meant 288 ng/400ul. Accordingly the content in the sample was 2.88 ug/ml. In analysis of intracerebral GABA by HPLC, in this experiment a reliable and possible value appears to be a concentration of about 2 ug/ml. However, a study is still being made on it. Less