Pharmacological study on intracerebral GABA mechanism

1. GABA免疫组化。(1)脊髓内GABA神经元的研究大鼠脊髓内GABA神经元发出黄绿色荧光。在脊髓前角和后角观察到CABA阳性hell和GABA阳性纤维的特异性荧光。为了比较脊髓内GABA的分布与合成酶的分布，我们使用GAD抗体通过间接荧光抗体技术制备样品，并研究相同水平上的分布状态。结果表明，GAD的分布与GABA的定位一致，但其荧光强度和分布密度均小于GABA的特异性荧光。因此，我们认为直接使用GABA抗体在组织化学中更准确;寻找GABA阳性细胞和阳性纤维。(2)脑内GABA神经元的研究我们用化学方法研究了黑质-纹状体GABA通路， 关于我们 与GAD定位比较，并与GAD定位比较。结果，GABA阳性细胞和阳性纤维在层中发出绿色荧光。在同一水平上研究GAD阳性细胞和阳性纤维的分布与GABA的分布基本一致。GABA阳性纤维在黑质内弥漫性发出绿色荧光. GABA阳性细胞在同一平面上的分布与GABA的分布一致.脑组织中GABA含量的生化分析。预处理后，将加入反应试剂的大鼠脑组织样品注入10- 25 μ l HPLC中。如果进样量超过25 μ l，峰宽可能变得太大，在254 nm 0.01 AUF处约为20-25 ng。脑组织样品通过标准曲线显示18 ng/25 μ l的值，保留时间为3.40分钟。总量为400 μ l，即288 ng/400 μ l。因此，样品中的含量为2.88 ug/ml。在通过HPLC分析脑内GABA中，在该实验中，可靠且可能的值似乎是约2 μ g/ml的浓度。然而，一项研究仍在进行。减

1. GABA immunohistochemistry. (1) Study of intraspinal GABA neuron The GABA neuron in the rat spinalcord issued yellowishgreen FITC-specific fluorescence. Specific fluorescence showing the localization of CABA-positive hells and GABA-positive fibers was observed in the spinal anterior horn and posterior horn. To compare the distribution of intraspinal GABA with that of a synthetic enzyme, we prepared specimens by an indirect fluorescent antibody technique using a GAD antibody, and studied for the distribution state on the same level. As a result, we found that the GAD distribution agreed with GABA localization, but the fluorescent intensity and distribution density were less than those of the specific fluorescence of GABA. Accordingly, we believe that using a GABA antibody directly is more accurate in histochemical ; searches for GABA-positive cells and positive fibers. (2) Study of intracerebral GABA neuron. We have studied immunohistochemically the nigra-striatal GABA pathway in the … More rat, and compared it with GAD localization, and compared it with GAD localization. As a result, GABA-positive cells and positive fibers issued green fluorescence in the stratum. Study of GAD-positive cells and positive fibers on the same level revealed that their distribution almost agreed with GABA distribution. GABA- positive fibers issued green fluorescence diffusedly at substantia nigra zoma compacta. The distribution of GAD-positive cells on the same level agreed with GABA distribution.2. Biochemical analysis of GABA content in brain tissues. After pretreatment, the rat brain tissue sample to which a reaction reagent had been added was injected into 10-25ul HPLC. The peak width may become too large if the injection amount exceeds 25ul, with about 20-25 ng at 254 nm 0.01 AUF. The brain tissue sample showed a value of 18 ng/25 ul by a standard curve, and the retention time was 3.40 minutes. The total amount was 400 ul, which meant 288 ng/400ul. Accordingly the content in the sample was 2.88 ug/ml. In analysis of intracerebral GABA by HPLC, in this experiment a reliable and possible value appears to be a concentration of about 2 ug/ml. However, a study is still being made on it. Less