Structural analysis of aldolase C gene and the identification of its expressed cell in brain

克隆了大鼠醛缩酶C基因。该基因由9个外显子和8个内含子组成。测定了该基因的核苷酸序列，包括801个核苷酸(NT)。5‘侧翼3590个新台币。基因区461个碱基。3‘侧翼序列。通过S1定位和引物延伸的方法确定了两个转录起始点。醛缩酶C基因只在脑中表达，在肝癌中也有部分表达。为了鉴定脑组织中表达醛缩酶C基因的细胞，我们用核探针对脑和脊髓进行了原位杂交。我们搜索了大脑皮层、小脑、海马体、丘脑。令人惊讶的是，银粒聚集在小脑的浦肯野细胞中，一些聚集在背根神经节和Nuc中。脊髓外侧运动肌。我们还测量了基因在基因组中的拷贝数。从Geme的测量和Southern blotting滴定，我们得出结论：每个大鼠单倍体基因组中存在一个大鼠醛缩酶C基因的单拷贝。

Rat aldolase C gene was isolated. The gene comprises 9 exons interruped by 8 introns. The nucleotide sequence of the gene was determined, which includes 801 nucleotides(nt.) of 5'flanking, 3590 nt. of the gene region and 461 nt. of 3' flanking sequences. Two transcriptional initiation sites were also determined by the method of S1 mapping and primer extension. Aldolase C mRNA was exclusively expressed in brain and some in hepatoma. To identify the cell expressing aldolase C mRNA in brain we performed in situ hypridization of brain and spinal cord using riboprobe. We searched cerebral cortex, cerebellum, hippocampus, thalamus of the brain. Surprisingly silver grains accumulated in the Purkinje cell of the cerebellum and some in dorsal root ganglion and Nuc. motorius lateralis of the spinal cord. We also measured the copy number of the gene in the genome. From the measurement of the geme, titrated by Southern blotting, we conclude that a single copy of the rat aldolase C gene occurs per rat haploid genome.