Pathological study on amyloidosis using of monoclonal antibodies against the synthesized peptides.

针对合成的人淀粉样蛋白A （AA）氨基酸序列中37 ~ 47个残基的短片段，制备了单克隆抗体KM-268。对牛、鼠、天鹅和人AA型淀粉样变性的T免疫组化方法可用于不同种类AA型淀粉样变性的鉴定。从合成的小鼠血清淀粉样蛋白A （SAA）氨基酸序列的多肽或残基中，制备了抗SAA蛋白单克隆抗体KM-503。免疫组化检测到小鼠肝细胞中的SAA蛋白，但未检测到各器官中的淀粉样蛋白沉积。尿管免疫反应阳性。这一发现表明SAA通过尿液过滤，并在尿小管上皮和miIII中被重新吸收。淀粉样蛋白增强因子（AEF） a)衰老小鼠的AEF；在9周龄小鼠脾匀浆中检测AEF活性。52周龄小鼠的上清液中存在较强的AEF活性。均质小鼠仅观察到弱活性，9周龄小鼠无活性。研究结果表明，AEF与衰老有关。b)中性粒细胞中AEF的免疫细胞化学证据；对AEF进行淀粉样变性刺激。制备了抗AEF血清，抗血清与刺激小鼠器官中性粒细胞反应强烈，与正常小鼠器官中性粒细胞反应弱。结果表明，AEF可能与中性粒细胞有关，且AEF在中性粒细胞中的活性随时间的增加而增加

O. Monoclonal antibody against AA protein A monoclonal antibody (KM-268) was raised against the synthesized shor to 37-47 residues in amino acid sequence of human amyloid A (AA) protein. T immunohistchemically to amyloid tissues from cow, mouse, swan and human AA was useful for the identification of AA type amyloidosis of various speciesII. Monoclonal antibody against SAA protein A monoclonal antibody (KM-503) was raised the synthesized peptides cor residues in amino acid sequence of murine serum amyloid A (SAA) protein. Thimmunohistochemically to SAA protein in the hepatocytes of mice after an am but not to the amyloid deposits in various organs. Positive immunoreaction the uriniferous tubles. The finding suggests that SAA is filterered through urine and is reabsorbed in the epithelium of the uriniferous tubule, and miIII. amyloid enhancing factor (AEF) a) AEF in aging mice; AEF activity was tested in spleen homogenates f and 9-week-old mice. Strong AEF activity was present in the supernatant of from the 52-week-old mice. Only weak activity was observed in the homogenat mice, and none was from the 9-week-old mice. The findings suggest that AEF with aging. b) An immunocytochemical evidence of AEF in neutrophils; AEF was extr recieved an amyloidogenic stimulation. An antiserum against AEF was raised antiserum reacted strongly with the neutrophils in the organs of mice with stimulation, and weakly in normal mice. The findings suggest that AEF might neutrophils, and that AEF activity in the neutrophils increases with the am