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Analysis of Thymic Microenvironment for T lymphocyte Development

T 淋巴细胞发育的胸腺微环境分析

基本信息

批准号：
62570225
负责人：
MIZUTANI Shuki
金额：
$ 1.54万
依托单位：
The National Children's Hospital, The National Children's Medical research Center
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570225/
关键词：
Lymphokine CALLA Antigen DQ52 IgH gene 胸腺上皮細胞 Tリンパ球初期分化分子機構 DQ52 5′H領域 lineage fidelity レトロウィルスベタター largeT遺伝子細胞質内CD3

项目摘要

In order to study the molecular mechanisms for early lymphocyte development, we have carried out the biochemical analysis of Common ALL antigen, which is expressed on the early stage of differentiation of B /or T lymphocyte. The result of our study indicates that the minor difference of CALLA molecule among differnt tissues is mainly due to those of syalidization.In order to clarify the correlation of the differentiation antigen expression and genome organization of Ig heavy chain gene, we have studied the molecular features in 78 ALL patients. As the results of this study, we found B precursor ALLs with germ line configuration of IgH gene, those with the rearrangement of Domega52 and those with the rearrangement of D 5' to Domega52. We propose a model for the classification of early B lymphocyte based upon these findings. Thymic epithelial cell lines were established by using gene transfer technique. SV40 ori- gene was introduced into fetal thymic epithelia. As the results 2 cell lines were established. They produced M-CSF and experessed LFA3 molecule which is a lignad for CD2.Our study is expected to help disclose the mechanisms for lymphocyte development in human.

为了研究早期淋巴细胞发育的分子机制，我们对B/或T淋巴细胞分化早期表达的常见ALL抗原进行了生化分析。我们的研究结果表明，不同组织中CALLA分子的微小差异主要是由于syliidization所致。为了阐明Ig重链基因的分化抗原表达与基因组组织的相关性，我们对78例ALL患者的分子特征进行了研究。作为本研究的结果，我们发现了具有 IgH 基因种系配置的 B 前体 ALL、具有 Domega52 重排的 B 前体 ALL 以及具有 D 5' 重排为 Domega52 的 B 前体 ALL。我们根据这些发现提出了早期 B 淋巴细胞分类的模型。采用基因转移技术建立胸腺上皮细胞系。将SV40 ori-基因引入胎儿胸腺上皮。结果建立了2个细胞系。他们产生了M-CSF并表达了LFA3分子，该分子是CD2的配体。我们的研究有望帮助揭示人类淋巴细胞发育的机制。