MOLECULAR FUNCTION OF DNA REPAIR FOR THE DEVELOPMENT

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ATM is a gene involved in the pathogenesis of Ataxia Telangiectasia and plays an important role in genome surveillance system. We found ATM mutation allow cells to override G2/M checkpoint in response to topoisomerase II (Topo II) inhibitor. Topo II inhibitor also cleaves MLL gene. These finding led us to investigate possible MLL translocation by Topo II inhibitor in ATM deficient cells. As the result we demonstrated frequent MLL translocation in these conditions by FISH analyses.Artemis is a molecule involved in the development of primary immune deficiency and phosphorylated by DNA-PKcs. We found ATM is phosphorylated at S645 in ATM dependent manner in response to XIR. These findings placed Artemtis at the crossing road between ATM and DNA-Pkcs.