Effect of Glomerular Capillary Wall Alteration on the Distribution of Immune Complex

肾小球毛细血管壁改变对免疫复合物分布的影响

• 批准号: 62570283
• 负责人: NAGASE Mitsumasa
• 金额: $ 1.15万
• 依托单位: First Department of Medicine, Teikyo University School of Medicine (1988)Hamamatsu University School of Medicine (1987)
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570283/
• 关键词: 免疫複合体; ダウノマイシン; 糸球体蛋白透過性; 蛋白尿; アポフェリチン; SLE; ループス腎炎

An investigation was performed to show whether an alteration of permselectivity of glomerular capillary wall (GCW) per se may influence the distribution of immune complex (IC) in the development of experimental ic nephritis. Apoferritin was used to induce IC nephritis in mice. To alter the permselectivity of GCW, daunomycin was injected to mice prior to the immunization with apoferritin. The results were compared between the animals in which apoferritin alone was given and those in which daunomycin and apoferritin were administered. In the former animals, the IgG and C_3 distribution was confined to mesangial area, thile the latter showed IgG and C_3 not only in mesangial area but also along the capillary wall.As expected, daunomycin administration induced increased urinary IgG and albumin excretion reflecting the enhanced macromolecular permselectivity in GCW. However, an alteration of antibody production or that of affinity of IC was not noted, which may be a factor other than the macromolecular permelectivity of GCW per se, responsible for the alteration of IC distribution. The findings suggest that charge of GCW per se can induce the althration of IC distribution in glomeruli, and may provide a clue for the future development of effective therapy for glomerulonephritis.

本文研究了肾小球毛细血管壁（GCW）选择性渗透性的改变是否影响免疫复合物（IC）在实验性IC肾炎中的分布。用脱铁铁蛋白诱导小鼠IC肾炎。为了改变GCW的选择透过性，在用脱铁铁蛋白免疫之前向小鼠注射柔红霉素。结果进行了比较的动物，其中单独给予脱铁铁蛋白和柔红霉素和脱铁铁蛋白的管理。结果表明，柔红霉素组IgG和C_3的分布仅限于系膜区，而柔红霉素组IgG和C_3不仅分布于系膜区，而且还分布于沿着毛细血管壁。然而，没有注意到抗体产生或IC亲和力的改变，这可能是GCW本身的大分子渗透性以外的因素，负责IC分布的改变。提示GCW本身的电荷可引起IC在肾小球内分布的改变，为进一步开发肾小球肾炎的有效治疗方法提供了线索。

项目成果

Mitsumasa Nagase: "Basement Membrane in Health Disease" Academic Press, 4 (1987)

长濑光政：《健康疾病中的基底膜》学术出版社，4（1987）

Mitsumasa Nagase: Electrical Charge and Immune Complex Deposition in GBM,in Basement Membrane in Health and Disease,. Academic Press, 405-409 (1987)

Mitsumasa Nagase：GBM 中的电荷和免疫复合物沉积，健康和疾病中的基底膜。

M. Kimura;M. Nagase;A. Hishida;N. Honda.: American Journal of Pathology.149-156 (1987)

M.木村;M.

Y.Fujigaki,;M.Nagase,;S.Kobayashi,;N.Honda.: Virch.Arch.Pathol.Anat.413. 159-165 (1988)

Y.Fujigaki，；M.Nagase，；S.Kobayashi，；N.Honda.：Virch.Arch.Pathol.Anat.413。

M.Nagase,;K.Sakakibara,;N.Honda,;Y.Takada,;A.Takada.: Contribution to Nephrology. 68. 141-148 (1988)

M.Nagase,;K.Sakakibara,;N.Honda,;Y.Takada,;A.Takada.：对肾脏病学的贡献。