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Role of adhesion morecule between glomerular epithelial cells and basement membrane, and cytoskeleton in proteinuria

肾小球上皮细胞与基底膜间的粘附细胞及细胞骨架在蛋白尿中的作用

基本信息

批准号：
10671008
负责人：
NAGASE Mitsumasa
金额：
$ 1.92万
依托单位：
Teikyo University
依托单位国家：
日本
项目类别：
Grant-in-Aid for Scientific Research (C)
财政年份：
1998
资助国家：
日本
起止时间：
1998 至 1999
项目状态：
已结题

来源：
https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-10671008/
关键词：
protein uria Nephritis glomerulus epithelial cell Basement membrane integrin ハイマン腎炎 PAN腎症馬杉腎炎 ED-1

项目摘要

Proteiuria is a hallmark of glomerulonephritis, and induces progressive renal insufficiency. It is believed that the mechanism of urinary protein excretion is the disorder of permeability of glomerular capillary wall, but its precise mechanism is still unclear. Recently hyperpermeability of glomerular capillary wall is due to the disorder of glomerular epithelial cell and glomerular basement membrane. It is widely known that the epithelial cells detach from basement membrane in proteiuric state. This may be due to somehow derangement of attachment between epithelial cells and basement membrane. Recently it is reported that integrin family plays an important role for keeping attachment of epithelial cell and basement membrane. In this report we describe the amount and status of integrin expression in glomeruli.PAN nephrosis and passive Heymann nephrosis were induced in rats and immunofluorescence was carried out on the kidney sections from PAN nephrosis at day 3 and 7, from Heymann nephrosis at day 4 and 7. The sections were observed by confocal laser microscopy and data were analyzed computerized image analyzer. The amount of integrin expression has increased at day 3 of PAN nephrosis, but the linear like expression diminished which is observed on normal rat kidney. At day 7 the linear like pattern recovered. On the section of Heymann nephrosis at day 4, the amount of its expression decreased and day 7 the glanular pattern was observed in the epithelial cells. These results strongly indicated the pattern of integrin expression at glomerular capillary walls is more important than its amount.

蛋白尿是肾小球肾炎的标志，并引起进行性肾功能不全。目前认为，尿蛋白排泄的机制是肾小球毛细血管壁通透性的紊乱，但其确切机制尚不清楚。近年来肾小球毛细血管壁通透性增高是由于肾小球上皮细胞和肾小球基底膜结构紊乱所致。众所周知，上皮细胞在蛋白尿状态下与基底膜分离。这可能是由于上皮细胞与基底膜之间的附着紊乱所致。近年来研究发现，整合素家族在维持上皮细胞与基底膜的粘附中起重要作用。在这份报告中，我们描述的数量和状态的整合素的表达在肾小球。PAN肾病和被动Heymann肾病诱导大鼠和免疫荧光进行了肾切片从PAN肾病在第3和7天，从Heymann肾病在第4和7天。用激光共聚焦显微镜观察切片，计算机图像分析仪分析数据。在PAN肾病的第3天，整合素的表达量增加，但在正常大鼠肾脏中观察到的线性样表达减少。在第7天，线性样图案恢复。在Heymann肾病第4天的切片上，其表达量减少，第7天在上皮细胞中观察到腺状图案。这些结果有力地表明整合素在肾小球毛细血管壁的表达模式比其数量更重要。