A study of phosphoprotein produced by Dunn osteosarcoma

邓恩骨肉瘤产生的磷蛋白的研究

• 批准号: 62570675
• 负责人: UDAGAWA Eiichi
• 金额: $ 1.22万
• 依托单位: Gunma University
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570675/
• 关键词: Phosphoproteins; Tumor markers; Osteosarcoma clone; 予後因子; アルカリフォスファターゼ; 骨芽細胞; クローン; 燐酸化; 石灰化

A phosphoprotein (MW=6,0000) was purified from conditioned medium, complete proteinfree (GM-40), of Dunn osteosarcoma. It showed alkaline phosphatase activity. From the data, it waw shown that one of the most prominent phosphoproteins secreted by osteosarcoma is alkaline phosphatase. No correlation between the prognosis of tumor bearing host, tumor doubling time (in vivo and in vitro), the incidence of pulmonary metastasis, and the level of alkaline phosphatase production. These phenomena was analysed theoretically using gene control system and the phenotypic diversity of the tumor. Under these conditions, Corelations between the prognosis of the patients and the level of tumor markers are only phenomenological, and tumor markers should be used to demonstrate the presence of the tumor elsewhere. Biologically, there is no reason to explain the relation between the two phenomena.

从Dunn骨肉瘤的条件培养基，完全无蛋白（GM-40）中纯化了一个磷酸化蛋白（MW=6 000）。显示碱性磷酸酶活性。结果表明，骨肉瘤分泌的最主要的磷酸化蛋白之一是碱性磷酸酶。荷瘤宿主的预后、肿瘤倍增时间（体内和体外）、肺转移发生率与碱性磷酸酶产生水平无相关性。利用基因控制系统和肿瘤表型多样性对这些现象进行了理论分析。在这种情况下，患者的预后与肿瘤标志物水平之间的相关性只是现象学上的，肿瘤标志物应该用来证明肿瘤在其他地方的存在。从生物学上讲，没有理由解释这两种现象之间的关系。

项目成果

Masaki Chigira et al.: "Bone metastasis as a non-stochastic process" Medical Hypotheses. (1989)

Masaki Chigira 等人：“骨转移是一个非随机过程”医学假设。

渡辺秀臣: 日本整形外科学会雑誌. 62. 369ー375 (1987)

渡边秀美：日本骨科学会杂志 62. 369-375 (1987)。

Masaki Chigira et al.: "Purification and characterization of an alkaline phosphatase secreted by Dunn osteosatcoma (clone 14)." J. Biological Chemistry.

Masaki Chigira 等人：“Dunn 骨肉瘤（克隆 14）分泌的碱性磷酸酶的纯化和表征。”

Masaki Chigira,et al.: Medical Hypotheses. (1989)

Masaki Chigira 等人：医学假设。

Masaki Chigira; Hideomi Watanbe: "Establishment of highly metastatic clones without tumorigenicity derived from Dunn osteosarcoma." Tumor Biology. 9. 293-300 (1988)

正崎奇吉拉；