An experimental study on the antigenicity of inorganic ceramics using as biomaterials.

无机陶瓷生物材料抗原性的实验研究。

• 批准号: 59570631
• 负责人: UDAGAWA Eiichi
• 金额: $ 0.9万
• 依托单位: Gunma University School of Medicine
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1984
• 资助国家: 日本
• 起止时间: 1984 至 1986
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-59570631/
• 关键词: Biomaterials; Ceramics; Antigenicity; Immunogenitics; Oxidation-reduction reaction; Loosening; Inflammation; 吸着

Significant footpad swelling was observed in mice immunized with tricalcium phosphate ceramic at 2 and 4 weeks after immunization. Antigenic specificity was demonstrated in cross-over design. Time course of the reaction suggest that delayed-type hypersensitivity played an important role. On the other hand, mice were died after administration of apatite-wollastonite glass ceramic. The death was induced by the ceramic powder itself, and was not induced by the supernatant of the powder. Significant footpad swelling showed the presence of delayed-type hypersensitivity against apatite-wallostonite. The adsorption behavior of cytochrome c on silica obeys the Langumuir adsorption irotherm. Furthermore, silica induced electron transfer of cytochrome c.

在免疫后2周和4周，用磷酸三钙陶瓷免疫的小鼠中观察到显著的足垫肿胀。在交叉设计中证明了抗原特异性。反应的时间进程表明迟发型超敏反应起了重要作用。另一方面，给药磷灰石-硅灰石玻璃陶瓷后，小鼠死亡。死亡是由陶瓷粉末本身诱导的，而不是由粉末的上清液诱导的。显著的足垫肿胀表明存在对磷灰石-硅灰石的迟发型超敏反应。细胞色素c在硅胶上的吸附符合Langumuir吸附等温式。此外，二氧化硅诱导细胞色素c的电子转移。

项目成果

Mitsuo Nagase,et al.: J.Orthop.Res.

Mitsuo Nagase 等人：J.Orthop.Res。

Mitsuo Nagase: "Antigenicity of alumina ceramic and calcium phosphate ceramics - Genetic control of the immune response -" J. Jpn. Orthop. Ass. (in Japanese). 59. 183-191 (1985)

Mitsuo Nagase：“氧化铝陶瓷和磷酸钙陶瓷的抗原性 - 免疫反应的遗传控制 -” J. Jpn.

長瀬満夫: 日本整形外科学会雑誌. 59. 183-191 (1985)

Mitsuo Nagase：日本骨科学会杂志 59. 183-191 (1985)。

Masaki Chigira,et al.: J.Orthop.Res.

Masaki Chigira 等人：J.Orthop.Res。

Mitsuo Nagase et al.: "Silica-induced oxidization of cytochrome c." J. Orthop. Res.

Mitsuo Nagase 等人：“二氧化硅诱导的细胞色素 c 氧化”。