Experimental study for the differntial conduction blockade of pain-mediating nerve fibers.

本研究旨在探讨局麻药地布卡因和镇痛药神经妥乐平对痛觉神经纤维的不同传导阻滞作用。观察了离体兔颈迷走神经在不同浓度地布卡因和神经妥乐平溶液中孵育时诱发复合动作电位振幅的变化。结果表明，3 × 10^ <-3>%和3 × 10^ <-2>%的西布卡因溶液对痛觉神经纤维的传导阻滞作用不可逆，而对痛觉神经纤维的传导阻滞作用不可逆。两种局麻药对痛觉神经纤维的传导阻滞作用均不可逆，但对痛觉神经纤维的传导阻滞作用不可逆。两种局麻药对痛觉神经纤维的传导阻滞作用均不可逆，而对痛觉神经纤维的传导阻滞作用均不可逆。两种局麻药对痛觉神经纤维的传导阻滞作用均不可逆，而对痛觉神经纤维的传导阻滞作用均不可逆神经妥乐平对α β和C成分均无传导阻滞作用，表明地布卡因和神经妥乐平均无选择性阻滞痛神经纤维的作用。虽然这些结果是我们在本研究中最初尝试的反向观察，但本研究中使用的方法被认为可用于研究体外神经纤维的差异性传导阻滞，我们正在用这种方法研究维生素B12和对羟基苯甲酸甲酯的作用。

The purpose of this study is to investigate the differential conduction blockade of pain-mediating nerve fibers by dibucaine, a kind of local anesthetics, and Neurotropin, an analgesic.Changes of the amplitudes of evoked compound action potentials elicited from excised rabbit cervical vagus nerve during incubation in solutions with various concentrations of each agent were observed.3*10^<-3>% and 3*10^<-2>% of sibucaine solution caused irreversible conduction blockade of abeta and C component respectively.Neurotropin did not cause any conduction blockade of both a beta and C components.These result indicate that neither dibucaine nor Neurotropin have an action to block pain-mediating nerve fibers selectively. Although these results were reverse observation which we attempted initially on this study, the method used on this study is thought tobe useful to investigate differential conduction blockade of nerve fibers in vitro.We are investigating the effect of Vitamin B12 and p-hydroxybenzoic acid methyl ester with this method.