An analysis of bladder carcinomas by flow-cytometry

膀胱癌的流式细胞术分析

• 批准号: 62570715
• 负责人: YACHIKU Sunao
• 金额: $ 1.22万
• 依托单位: Asahikawa Medical College
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570715/
• 关键词: Flow-Cytometry; DNA; Bladder Carcinoma; フローサイトメトリー

An analysis of DNA ploidy of bladder transitional cell carcinomas by flow-cytometry was performed. The correlation of various tumor factors (e.g. tumor grade, tumor stage, etc.) and the DNA ploidy was analyzed.1. Tumor grade and DNA ploidy : 72 samples were analyzed, and 31 samples(43%) were non-diploid. 2 of 6 samples(33%) of the grade 1 tumors 6 of 17(35%) of the grade 2, and 23 of 49(47%) of the grade 3 were non- diploid.2. Tumor stage and DNA ploidy : The pathological stage on the specimen of total eystectomy and DNA ploidy were analyzed. The percentages of non-diploid pattern were 100%(1/1) in Ta, 60%(3/5) in T1a, 38%(3/8) in T1b, 63%(5/8) in T2, 40%(4/10) in T3a, 43%(3/7) in T3b, and 50%(I/2) in T4 carcinomas.3. So called downstaging by preoperative irradiation and DNA ploidy : The downstaging was observed in 7 cases(35%) of diploid cases, and 4 cases(36%) of non-diploid cases.4. The change of the pattern of DNA ploidy by irradiation : No significant change of the pattern of DNA ploidy was seen before and after irradiation.5. Prognosis and DNA ploidy : in the cases of tumor-free-survival over 3 years the DNA pattern was non-diploid in 8 cases(67%), and in the cases of cancer-death it was non-diploid in 7 cases(64%).Above mentioned data revealed no significant correlation between DNA ploidy and the various conventional factors and prognoses of the bladder carcinomas.

应用流式细胞术对膀胱移行细胞癌进行DNA倍体分析。各种肿瘤因素（如肿瘤分级、肿瘤分期等）的相关性并进行DNA倍性分析.肿瘤分级和DNA倍体：分析了72份样本，31份样本（43%）为非二倍体。1级肿瘤6例中有2例（33%）为非二倍体，2级肿瘤17例中有6例（35%）为非二倍体，3级肿瘤49例中有23例（47%）为非二倍体.肿瘤分期和DNA倍体：分析总细胞数和DNA倍体标本的病理分期。非二倍体型在Ta组为100%（1/1），T1 a组为60%（3/5），T1 b组为3/8%（3/8），T2组为63%（5/8），T3 a组为40%（4/10），T3 b组为43%（3/7），T4组为50%（1/2）.所谓的术前放疗和DNA倍体降低分期：二倍体7例（35%），非二倍体4例（36%）.辐射对DNA倍体模式的影响：辐射前后DNA倍体模式无明显变化.预后和DNA倍性：无瘤生存3年以上的病例中，有8例（67%）DNA是非二倍体，癌死亡病例中，有7例（64%）DNA是非二倍体，上述数据表明DNA倍性与膀胱癌的各种常规因素和预后无明显相关性。