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Studies on the endotoxinshock and hypoxic pulmonary vasoconstriction by biomicroscopical observation.

内毒素休克和缺氧性肺血管收缩的生物显微镜观察研究。

基本信息

批准号：
62570700
负责人：
NAKAJIMA Kazuo
金额：
$ 1.02万
依托单位：
Fukui Medical School.
依托单位国家：
日本
项目类别：
Grant-in-Aid for General Scientific Research (C)
财政年份：
1987
资助国家：
日本
起止时间：
1987 至 1988
项目状态：
已结题

项目摘要

1. ObjectIn oder to study of endotoxin shock and hypoxic condition on the microcirculation, we observed continuously the mesentrium of Wistar strain rats under a transmitting microscope with an irrigation system.2. Method(1) Recording the images of the mesenterium on the high speed video, and measuring the velocity of blood and the diameters of vessels. (2) Measuring the continuous change of the diameters of microvessels using an image splitter, and recording on a polygraph. Also measuring and recording the arterial pressure.3. ResultsIn normal rats as control, the intravenous injection of urinasttin (UST); anti-shock agent, casued shortening of vasoconstrictive periods induced by topical norepinephrine(NE) of 1 g. These shortening were notable during the recovery periods. In cont-rast with this, in the rats of endotoxin shock, UST caused less shortening of recovery periods. So we supposed that USt may affect anything to the abnormal vascular action with endotoxin.The rats under endotoxin shock with UST treatment kept more higher arterial pressure than not treated ones. These facts were co-operated with the results of the change in the diameter of microarteriole.Next, we compared the two groups of rats under endotoxin shock. One group rats were treated by UST, before endotoxin injection, the others were treated by UST after endotoxin. The former kept high arterial pressure and blood velocity, although vasoconstriction of mesenterium was less than the latter. From above results, we concluded that pre-treatment by UST softened the symptoms of endotoxin shock.Under hypoxic condition (F_<102>=0.15), the micro-circulation of mesenterial vessels of rats under endotoxin shock was extended of its diameter of vessels gradually, and became to constract more sensitively by topical of NE(1 g), and the hypoxic vasoconstriction was also promoted during the endotoxin shock.

1.目的为研究内毒素休克及缺氧对微循环的影响，在带灌流系统的透射显微镜下，对Wistar系大鼠的系膜进行连续观察。方法(1)在高速录像机上记录肠系膜的图像，测量血流速度和血管直径。(2)用图像分离器测量微血管直径的连续变化，并记录在测谎仪上。同时测量并记录动脉压。结果正常大鼠静脉注射乌司他丁(UST)、抗休克药后，局部注射1g去甲肾上腺素(NE)引起的血管收缩时间明显缩短，且在恢复期明显缩短。与此相反，在内毒素休克大鼠中，UST引起的恢复期缩短较少。因此，我们推测UST可能对内毒素引起的血管异常作用有一定影响。在内毒素休克条件下，UST治疗的大鼠比未治疗的大鼠维持更高的动脉压。这些事实与微动脉直径变化的结果相吻合。接下来，我们比较了两组大鼠在内毒素休克下的表现。一组在注射内毒素前给予UST治疗，另一组在注射内毒素后给予UST治疗。前者维持较高的动脉压和血流速度，但肠系膜血管收缩较后者小。在低氧条件下(F&lt；102&gt；=0.15)，内毒素休克大鼠肠系膜血管微循环血管管径逐渐扩大，局部应用NE(1g)使微循环变得更加敏感，在内毒素休克过程中也促进了缺氧性血管收缩。