Studies on the endotoxinshock and hypoxic pulmonary vasoconstriction by biomicroscopical observation.

1. 目的研究内毒素休克和缺氧情况对Wistar系大鼠微循环的影响，在灌洗系统下用透射显微镜连续观察肠系膜。方法(1)在高速视频上记录肠系膜图像，测量血流速度和血管直径。(2)利用图像分配器测量微血管直径的连续变化，并在测谎仪上记录。同时测量并记录动脉压。结果以正常大鼠为对照，静脉注射尿司他丁（UST）；抗休克剂，可缩短外用1 g去甲肾上腺素（NE）引起的血管收缩期。这种缩短在恢复期是显著的。与此相反，在内毒素休克大鼠中，UST引起的恢复期缩短较少。因此，我们推测USt可能与血管内毒素异常作用有关。内毒素休克后经UST处理的大鼠动脉压高于未处理的大鼠。这些结果与微动脉直径变化的结果相吻合。接下来，我们比较两组内毒素休克大鼠。一组大鼠在内毒素注射前先进行UST处理，另一组大鼠在内毒素注射后进行UST处理。前者保持较高的动脉压和血流速度，但肠系膜血管收缩小于后者。从以上结果，我们得出结论，UST预处理可以减轻内毒素休克的症状。在缺氧条件下（F_<102>=0.15），内毒素休克大鼠肠系膜血管微循环血管直径逐渐扩大，外用NE（1 g）后血管收缩更加敏感，内毒素休克时血管缺氧收缩也得到促进。

1. ObjectIn oder to study of endotoxin shock and hypoxic condition on the microcirculation, we observed continuously the mesentrium of Wistar strain rats under a transmitting microscope with an irrigation system.2. Method(1) Recording the images of the mesenterium on the high speed video, and measuring the velocity of blood and the diameters of vessels. (2) Measuring the continuous change of the diameters of microvessels using an image splitter, and recording on a polygraph. Also measuring and recording the arterial pressure.3. ResultsIn normal rats as control, the intravenous injection of urinasttin (UST); anti-shock agent, casued shortening of vasoconstrictive periods induced by topical norepinephrine(NE) of 1 g. These shortening were notable during the recovery periods. In cont-rast with this, in the rats of endotoxin shock, UST caused less shortening of recovery periods. So we supposed that USt may affect anything to the abnormal vascular action with endotoxin.The rats under endotoxin shock with UST treatment kept more higher arterial pressure than not treated ones. These facts were co-operated with the results of the change in the diameter of microarteriole.Next, we compared the two groups of rats under endotoxin shock. One group rats were treated by UST, before endotoxin injection, the others were treated by UST after endotoxin. The former kept high arterial pressure and blood velocity, although vasoconstriction of mesenterium was less than the latter. From above results, we concluded that pre-treatment by UST softened the symptoms of endotoxin shock.Under hypoxic condition (F_<102>=0.15), the micro-circulation of mesenterial vessels of rats under endotoxin shock was extended of its diameter of vessels gradually, and became to constract more sensitively by topical of NE(1 g), and the hypoxic vasoconstriction was also promoted during the endotoxin shock.