Studies on the endotoxinshock and hypoxic pulmonary vasoconstriction by biomicroscopical observation.

1.目的在透射显微镜下连续观察Wistar系大鼠肠系膜微循环，探讨内毒素休克和缺氧对微循环的影响.方法（1）在高速摄像机上记录肠系膜图像，测量血流速度和血管直径。(2)用分像仪测量微血管直径的连续变化，并记录在多导生理记录仪上。同时测量并记录动脉压。结果正常大鼠静脉注射乌司他丁（UST），抗休克药可明显缩短去甲肾上腺素（NE）引起的血管收缩时间。这些缩短在恢复期间是显著的。而内毒素休克大鼠，UST对恢复期的缩短作用较小。因此，我们推测USt可能对内毒素引起的异常血管活动有一定的影响，内毒素休克大鼠经USt治疗后，动脉压明显高于未治疗组。这些结果与微动脉直径变化的结果相一致。其次，我们比较了内毒素休克的两组大鼠。一组大鼠在注射内毒素前给予超声心动图治疗，另一组大鼠在注射内毒素后给予超声心动图治疗。前者维持较高的动脉压和血流速度，但肠系膜血管收缩较后者轻。结果表明，UST预处理可减轻内毒素休克的症状，在缺氧条件下（F_<102>=0.15），内毒素休克大鼠肠系膜血管微循环逐渐扩张，对NE（1g）的局部应用更为敏感，并促进内毒素休克时的缺氧性血管收缩。

1. ObjectIn oder to study of endotoxin shock and hypoxic condition on the microcirculation, we observed continuously the mesentrium of Wistar strain rats under a transmitting microscope with an irrigation system.2. Method(1) Recording the images of the mesenterium on the high speed video, and measuring the velocity of blood and the diameters of vessels. (2) Measuring the continuous change of the diameters of microvessels using an image splitter, and recording on a polygraph. Also measuring and recording the arterial pressure.3. ResultsIn normal rats as control, the intravenous injection of urinasttin (UST); anti-shock agent, casued shortening of vasoconstrictive periods induced by topical norepinephrine(NE) of 1 g. These shortening were notable during the recovery periods. In cont-rast with this, in the rats of endotoxin shock, UST caused less shortening of recovery periods. So we supposed that USt may affect anything to the abnormal vascular action with endotoxin.The rats under endotoxin shock with UST treatment kept more higher arterial pressure than not treated ones. These facts were co-operated with the results of the change in the diameter of microarteriole.Next, we compared the two groups of rats under endotoxin shock. One group rats were treated by UST, before endotoxin injection, the others were treated by UST after endotoxin. The former kept high arterial pressure and blood velocity, although vasoconstriction of mesenterium was less than the latter. From above results, we concluded that pre-treatment by UST softened the symptoms of endotoxin shock.Under hypoxic condition (F_<102>=0.15), the micro-circulation of mesenterial vessels of rats under endotoxin shock was extended of its diameter of vessels gradually, and became to constract more sensitively by topical of NE(1 g), and the hypoxic vasoconstriction was also promoted during the endotoxin shock.