Study on the Role of Prolactin in Reproductive Physiology.

催乳素在生殖生理学中作用的研究。

• 批准号: 62570744
• 负责人: TAKETANI Yuji
• 金额: $ 1.15万
• 依托单位: The University of Tokyo
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1989
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570744/
• 关键词: Prolactin; gonadotropin; gonadotropin-releasing hormone(GnRH); hypothalamus; anterior pituitary gland; endometriosis; inadequate luteal phase; infertility; 下垂体前葉; ゴナドトロピン; 母乳量; TRHテスト; ブロモクリプチン; LHRHテスト; LH; 高プロラクチン血症; FSH; LHRH

Modulatory effects of prolactin(PRL) on gonadotropin release were examined. An acute administration of OPRL to ovariectomized rats induced no influence on the content of gonadotropin-releasing hormone(GnRH) in the hypothalamus. However, the release of GnRH and gonadotropin were inhibited in rats given OPRL. The reduction in the gonadotropin release seems to be ascribable to the suppression of GnRH release. An acute elevation in PRL levels did not affect the pituitary responsiveness to GnRH. Thus, we deduced from these results that hyperprolactinemia inhibits the release of gonadotropin by primarily impairing the release of GnRH from the hypothalamus.Then, the action of PRL on the secretion of donadotropin was investigated using a primary cell culture system of rat anterior pituitary gland. LH release was increased by adding PRL into culture media in a dose-dependent manner in the range between 10-1000 ng/ml. The stimulatory effect of PRL was further augmented in the presence of GnRH an … More d estradiol. PRL also caused an increase in FSH release. These data demonstrate that PRL stimulates the release of gonadotropin at the pituitary level, suggesting a paracrine mode of action of PRL in the anterior pituitary gland.Clinically, the synchronous secretion of LH and PRL is frequently observed in normal cycling and hypogonadal women. Looking for the effects of GnRH on the PRL release, GnRH was given to women with normal menstruation and various menstrual disorders. This study offered such conclusion that GnRH occasionally alters the blood levels of PRL in either positive or negative manner depending on basal PRL levels.In order seek the underlying mechanism of infertility associated with endometriosis, we demonstrate a close linkage of endometriosis and inadequate luteal function as evidenced by lower levels of progesterone at midluteal phase. There appears to be no correlation between the levels of progesterone and PRL. Therefore, conclusion is drawn that an aberrant secretion of PRL has no relation to the impaired corpus luteum function in endometriosis. Less

观察催乳素(PRL)对促性腺激素释放的调节作用。卵巢切除大鼠急性给予OPRL对下丘脑促性腺激素释放激素(GnRH)含量无影响。但OPRL对大鼠促性腺激素释放和促性腺激素释放有抑制作用。促性腺激素释放的减少似乎归因于对GnRH释放的抑制。PRL水平的急剧升高并不影响垂体对GnRH的反应性。根据这些结果，我们推断高催乳素血症主要通过影响下丘脑GnRH的释放来抑制促性腺激素的释放。然后，利用大鼠垂体前叶原代细胞培养系统研究了PRL对促性腺激素分泌的影响。促黄体生成素在10-1000 ng/ml范围内呈剂量依赖性增加，促性腺激素释放激素和…对促黄体生成素的刺激作用进一步增强更多的雌二醇。PRL还引起FSH释放增加。这些数据表明，PRL在垂体水平刺激促性腺激素的释放，提示PRL在垂体前腺以旁分泌方式起作用。临床上，在正常周期和性腺功能低下的妇女中经常观察到促黄体生成素和催乳素的同步分泌。为了解促性腺激素释放激素对催乳素释放的影响，对月经正常和各种月经紊乱的妇女给予促性腺激素释放激素。本研究得出这样的结论：促性腺激素释放激素偶尔会以正性或负性的方式改变血中催乳素水平，这取决于基础催乳素水平。为了探讨子宫内膜异位症不孕的潜在机制，我们证明子宫内膜异位症与黄体功能低下密切相关，黄体中期孕酮水平降低就是明证。孕酮水平与催乳素水平之间似乎没有相关性。结论：子宫内膜异位症患者体内PRL异常分泌与黄体功能受损无关。较少

项目成果

Yoji Takatani: "Effect of prolaction administration on LH,FSH and GnRH in ovaniectomized rats" Role of Prolactin in Human Reproduction eds.Mizuno et al.(Karger). 91-100 (1988)

Yoji Takatani：“催乳剂给药对卵巢切除大鼠中 LH、FSH 和 GnRH 的影响”催乳素在人类生殖中的作用编辑。Mizuno 等人（Karger）。

Koji Kugu: "Stimulatory action of prolactin on gonadotropin secretion in vitro." Endocrinol,Japon.36. 509-514 (1989)

Koji Kugu：“催乳素对体外促性腺激素分泌的刺激作用。”

Koji Kugu, Yuji Taketani and Masahiko Mizuno: "Changes in prolactin levels caused by luteinizing hormone releasing hormone." Endocrinol. Japon. 35(4) 545-548, 1988.

Koji Kugu、Yuji Taketani 和 Masahiko Mizuno：“黄体生成素释放激素引起的催乳素水平变化。”

Yuji,Taketani.;Masahiko,Mizuno.: Ichinoe et al(eds),Preservation of Tuboovarian Function in Gynecologic Be nign and Malignant Diseases(Raven Press). 559-567 (1988)

Yuji，Taketani.；Masahiko，Mizuno.：Ichinoe 等人（编辑），妇科良性和恶性疾病中输卵管卵巢功能的保留（Raven Press）。

Koji Kugu: "Danazol stimutates the release of follicle-stimulating hormone and inhibits the release of prolactin in vitro studies on rat pituitary cell culture." Arch-Gynecol.Obstet.244. 157-162 (1989)

Koji Kugu：“在大鼠垂体细胞培养物的体外研究中，达那唑刺激促卵泡激素的释放并抑制催乳素的释放。”