EFFECTS OF TUMOR PROMOTING AGENTS ON THE MALIGNANT TRANSFORMATION OF HYDATIDIFORM MOLE CELLS IN CULTURE

促癌剂对培养的葡萄胎细胞恶性转化的影响

• 批准号: 62570770
• 负责人: KENJO Takiko
• 金额: $ 1.22万
• 依托单位: TOKAI UNIVERSITY
• 依托单位国家: 日本
• 项目类别: Grant-in-Aid for General Scientific Research (C)
• 财政年份: 1987
• 资助国家: 日本
• 起止时间: 1987 至 1988
• 项目状态: 已结题
• 来源: https://kaken.nii.ac.jp/grant/KAKENHI-PROJECT-62570770/
• 关键词: PROMOTION; TPA; TLC; VITAMIN D; IN VITRO COLONY ASSAY; TRANSFORMATION; XENOCENIC TRANSPLANTATION; 培養細胞

SINCE THE ESTABLISHMENT OF THE TWO STAGE CARCINOGENESIS, VARIOUS TUMOR PROMOTERS HAVE BEEN REPORTED. EFFECTS OF TUMOR PROMOTING AGENTS ON THE MALIGNANT TRANSFORMATION OF THE HYDATIDIFORM MOLE CELLS ( BM-34 ) MAINTAINED FOR OVER TWO YEARS IN CULTURE, WERE EXAMINED.IN VITRO COLONY ASSAY SYSTEM IN SOFT AGAR AND XENOGENIC TRANSPLANTATION METHOD WERE USED TO PROVE WHETHER MALIGNANT TRANSFORMATION OF HYDATIDIFORM MOLE CELLS TREATED WITH THE PROMOTERS ( TPA: 12-O-TETRADECANOYL-PHORBOL-13-ACETATE ), ( TLC: TELEOCIDIN ), ( VITAMIN D DERIVATIVES : 1ALPHA,25-(OH)_2 D_3), HAD OCCURRED. THE HYDATIDIFORM MOLE CELLS WERE TREATED WITH TPA (0.1,1.0,10,100 ng/ml), TLC (1.0,10 ng/ml), VITAMIN (0.1,1.0,10 hg/ml) FOR VARIOUS LENGTH OF TIME (8,10,12,16,20 WEEKD).WHEN THE TREATED CELLS WERE SEEDED IN SOFT AGAR UNDER PRESENT TREATMENT CONDITION WITH TPA, TLC AND VITAMIN D , THE PLATING EFFICIENCY OF HYDATIDIFORM MOLE CELLS TREATED WITH TPA AND TLC FOR OVER 12 WEEKS INCREASED SIGNIFICANTLY IN COMPARISON WITH CONTROL, WHEREAS THAT OF THE CELLS TREATED WITH VITAMIN D FOR 12 WEEKS FROPPED IN INVERSE PROPORTION TO EACH CONCENTRATION OF THE DRUG.IN ADDITION, WE DID NOT OBTAIN ANY PROGRESSIVELY GROWING TUMOR EVEN IF WE HAD ATTEMPTED TO TRANSPLANT THESE CELLS INTO NUDE MICE.

自从两阶段致癌作用确立以来，已有多种肿瘤促进剂被报道。考察了促瘤剂对培养两年以上的葡萄胎细胞(BM-34)恶性转化的影响，并采用软琼脂体外集落分析系统和异种移植方法来验证其是否存在。用启动子（TPA：12-O-十四烷酰基-佛波醇-13-乙酸酯）处理的葡萄胎细胞的恶性转化 ), ( TLC: TELEOCIDIN ), ( 维生素 D 衍生物 : 1ALPHA,25-(OH)_2 D_3), 已经发生。葡萄胎细胞用 TPA (0.1,1.0,10,100 ng/ml)、TLC (1.0,10 ng/ml)、维生素 (0.1,1.0,10 hg/ml) 处理不同时间长度（8、10、12、16、20 周）。在当前处理条件下用 TPA、TLC 和维生素将种子接种到软琼脂中 D，用TPA和TLC处理超过12周的葡萄胎细胞的铺板效率与对照相比显着增加，而用维生素D处理12周的细胞的铺板效率与每个药物浓度成反比下降。此外，即使我们尝试将这些细胞移植到裸鼠体内，我们也没有获得任何逐渐生长的肿瘤。