EFFECTS OF TUMOR PROMOTING AGENTS ON THE MALIGNANT TRANSFORMATION OF HYDATIDIFORM MOLE CELLS IN CULTURE

自从两阶段致癌学说建立以来，各种肿瘤促进剂相继被报道。为探讨促癌剂对体外培养的葡萄胎细胞(BM-34)恶性转化的影响，采用体外软琼脂集落分析系统和异种移植方法，验证了促癌剂(TPA：12-O-四氢-佛波醇-13-乙酸酯)、(TLC：TELEOCIDIN)、(维生素D衍生物：1α，25-(OH)_2、D_3)对葡萄胎细胞的恶性转化作用。用TPA(0.1、1.0、10、100 ng/ml)、TLC(1.0、10 ng/ml)、维生素(0.1、1.0、10 HG/ml)处理葡萄胎细胞不同时间(8、10、12、16、20WEEKD)。在软琼脂中加入TPA、TLC和维生素D处理12周以上的葡萄胎细胞，与对照组相比，葡萄胎细胞的增殖效率显著提高，而维生素D处理12周的葡萄胎细胞，与DRUG的各浓度成反比。即使我们试图将这些细胞移植到裸鼠体内，我们也没有获得任何渐进性生长的肿瘤。

SINCE THE ESTABLISHMENT OF THE TWO STAGE CARCINOGENESIS, VARIOUS TUMOR PROMOTERS HAVE BEEN REPORTED. EFFECTS OF TUMOR PROMOTING AGENTS ON THE MALIGNANT TRANSFORMATION OF THE HYDATIDIFORM MOLE CELLS ( BM-34 ) MAINTAINED FOR OVER TWO YEARS IN CULTURE, WERE EXAMINED.IN VITRO COLONY ASSAY SYSTEM IN SOFT AGAR AND XENOGENIC TRANSPLANTATION METHOD WERE USED TO PROVE WHETHER MALIGNANT TRANSFORMATION OF HYDATIDIFORM MOLE CELLS TREATED WITH THE PROMOTERS ( TPA: 12-O-TETRADECANOYL-PHORBOL-13-ACETATE ), ( TLC: TELEOCIDIN ), ( VITAMIN D DERIVATIVES : 1ALPHA,25-(OH)_2 D_3), HAD OCCURRED. THE HYDATIDIFORM MOLE CELLS WERE TREATED WITH TPA (0.1,1.0,10,100 ng/ml), TLC (1.0,10 ng/ml), VITAMIN (0.1,1.0,10 hg/ml) FOR VARIOUS LENGTH OF TIME (8,10,12,16,20 WEEKD).WHEN THE TREATED CELLS WERE SEEDED IN SOFT AGAR UNDER PRESENT TREATMENT CONDITION WITH TPA, TLC AND VITAMIN D , THE PLATING EFFICIENCY OF HYDATIDIFORM MOLE CELLS TREATED WITH TPA AND TLC FOR OVER 12 WEEKS INCREASED SIGNIFICANTLY IN COMPARISON WITH CONTROL, WHEREAS THAT OF THE CELLS TREATED WITH VITAMIN D FOR 12 WEEKS FROPPED IN INVERSE PROPORTION TO EACH CONCENTRATION OF THE DRUG.IN ADDITION, WE DID NOT OBTAIN ANY PROGRESSIVELY GROWING TUMOR EVEN IF WE HAD ATTEMPTED TO TRANSPLANT THESE CELLS INTO NUDE MICE.